Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   I5154_RS26290 Genome accession   NZ_CP060242
Coordinates   5632838..5633434 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain B-I-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5627838..5638434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5154_RS26270 (I5154_005393) aroK 5628969..5629487 (-) 519 WP_003095833.1 shikimate kinase AroK -
  I5154_RS26275 (I5154_005394) pilQ 5629499..5631643 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  I5154_RS26280 (I5154_005395) comL/pilP 5631697..5632221 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  I5154_RS26285 (I5154_005396) pilO 5632218..5632841 (-) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  I5154_RS26290 (I5154_005397) pilN 5632838..5633434 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  I5154_RS26295 (I5154_005398) pilM 5633434..5634240 (-) 807 Protein_5206 type IV pilus assembly protein PilM -
  I5154_RS26305 (I5154_005400) pilM 5635477..5635737 (-) 261 Protein_5208 pilus assembly protein PilM -
  I5154_RS26310 (I5154_005401) - 5635922..5638393 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=413929 I5154_RS26290 WP_003095839.1 5632838..5633434(-) (pilN) [Pseudomonas aeruginosa strain B-I-1]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=413929 I5154_RS26290 WP_003095839.1 5632838..5633434(-) (pilN) [Pseudomonas aeruginosa strain B-I-1]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369