Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   I5102_RS25425 Genome accession   NZ_CP060240
Coordinates   5415978..5416403 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain G-I-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5410978..5421403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5102_RS25410 (I5102_005212) pilX 5411542..5412129 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  I5102_RS25415 (I5102_005213) pilY1 5412141..5415632 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  I5102_RS25420 (I5102_005214) pilY2 5415634..5415981 (+) 348 WP_012614546.1 type 4a fimbrial biogenesis protein PilY2 -
  I5102_RS25425 (I5102_005215) comF 5415978..5416403 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  I5102_RS25430 (I5102_005216) ispH 5416450..5417394 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  I5102_RS25435 (I5102_005217) fkpB 5417480..5417920 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  I5102_RS25440 (I5102_005218) lspA 5417913..5418422 (-) 510 WP_003094728.1 signal peptidase II -
  I5102_RS25445 (I5102_005219) ileS 5418415..5421246 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=413730 I5102_RS25425 WP_003094721.1 5415978..5416403(+) (comF) [Pseudomonas aeruginosa strain G-I-1]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=413730 I5102_RS25425 WP_003094721.1 5415978..5416403(+) (comF) [Pseudomonas aeruginosa strain G-I-1]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383