Detailed information NTG0412454

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GTF74_RS01300
• Genome accession: NZ_CP047303
• Coordinates: 276799..277815 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 biovar El Tor strain E7946
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 271799..282815

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF74_RS01285 (GTF74_01285)	-	272341..273072 (-)	732	WP_000495223.1	glutathione peroxidase	-
GTF74_RS01290 (GTF74_01290)	oxyR	273212..274105 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
GTF74_RS01295 (GTF74_01295)	-	274179..276656 (-)	2478	WP_000668733.1	penicillin-binding protein 1A	-
GTF74_RS01300 (GTF74_01300)	pilM	276799..277815 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
GTF74_RS01305 (GTF74_01305)	pilN	277805..278389 (+)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
GTF74_RS01310 (GTF74_01310)	pilO	278382..278972 (+)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
GTF74_RS01315 (GTF74_01315)	pilP	278962..279480 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
GTF74_RS01320 (GTF74_01320)	pilQ	279506..281221 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ family protein	Machinery gene
GTF74_RS01325 (GTF74_01325)	aroK	281408..281932 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=412454 GTF74_RS01300 WP_000520444.1 276799..277815(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain E7946]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=412454 GTF74_RS01300 WP_000520444.1 276799..277815(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485