Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   H5022_RS26400 Genome accession   NZ_CP060004
Coordinates   5618862..5619287 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain WK172     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5613862..5624287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5022_RS26385 (H5022_26245) pilX 5614426..5615013 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  H5022_RS26390 (H5022_26250) pilY1 5615025..5618516 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  H5022_RS26395 (H5022_26255) pilY2 5618518..5618865 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  H5022_RS26400 (H5022_26260) comF 5618862..5619287 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  H5022_RS26405 (H5022_26265) ispH 5619334..5620278 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  H5022_RS26410 (H5022_26270) fkpB 5620364..5620804 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  H5022_RS26415 (H5022_26275) lspA 5620797..5621306 (-) 510 WP_003102615.1 signal peptidase II -
  H5022_RS26420 (H5022_26280) ileS 5621299..5624130 (-) 2832 WP_003123398.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=412337 H5022_RS26400 WP_003094721.1 5618862..5619287(+) (comF) [Pseudomonas aeruginosa strain WK172]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=412337 H5022_RS26400 WP_003094721.1 5618862..5619287(+) (comF) [Pseudomonas aeruginosa strain WK172]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment