Detailed information NTG0412311

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GTF73_RS01325
• Genome accession: NZ_CP047297
• Coordinates: 281970..282986 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 biovar El Tor strain C6709
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 276970..287986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF73_RS01310 (GTF73_01310)	-	277512..278243 (-)	732	WP_000495223.1	glutathione peroxidase	-
GTF73_RS01315 (GTF73_01315)	oxyR	278383..279276 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
GTF73_RS01320 (GTF73_01320)	-	279350..281827 (-)	2478	WP_000668733.1	penicillin-binding protein 1A	-
GTF73_RS01325 (GTF73_01325)	pilM	281970..282986 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
GTF73_RS01330 (GTF73_01330)	pilN	282976..283560 (+)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
GTF73_RS01335 (GTF73_01335)	pilO	283553..284143 (+)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
GTF73_RS01340 (GTF73_01340)	pilP	284133..284651 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
GTF73_RS01345 (GTF73_01345)	pilQ	284677..286392 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ family protein	Machinery gene
GTF73_RS01350 (GTF73_01350)	aroK	286579..287103 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=412311 GTF73_RS01325 WP_000520444.1 281970..282986(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain C6709]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=412311 GTF73_RS01325 WP_000520444.1 281970..282986(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain C6709]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485