Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   H5J27_RS27635 Genome accession   NZ_CP059995
Coordinates   5809135..5809560 (+) Length   141 a.a.
NCBI ID   WP_033976378.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY3045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5804135..5814560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5J27_RS27620 (H5J27_27620) pilX 5804705..5805292 (+) 588 WP_033972921.1 type 4a pilus minor pilin PilX -
  H5J27_RS27625 (H5J27_27625) pilY1 5805304..5808789 (+) 3486 WP_033976379.1 type 4a pilus biogenesis protein PilY1 -
  H5J27_RS27630 (H5J27_27630) pilY2 5808791..5809138 (+) 348 WP_033972924.1 type 4a fimbrial biogenesis protein PilY2 -
  H5J27_RS27635 (H5J27_27635) comF 5809135..5809560 (+) 426 WP_033976378.1 type 4a pilus minor pilin PilE Machinery gene
  H5J27_RS27640 (H5J27_27640) ispH 5809607..5810551 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  H5J27_RS27645 (H5J27_27645) fkpB 5810637..5811077 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  H5J27_RS27650 (H5J27_27650) lspA 5811070..5811579 (-) 510 WP_025271359.1 signal peptidase II -
  H5J27_RS27655 (H5J27_27655) ileS 5811572..5814403 (-) 2832 WP_042168473.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15311.36 Da        Isoelectric Point: 10.0198

>NTDB_id=412235 H5J27_RS27635 WP_033976378.1 5809135..5809560(+) (comF) [Pseudomonas aeruginosa strain NY3045]
MRTRQKGFTLLEMVMVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=412235 H5J27_RS27635 WP_033976378.1 5809135..5809560(+) (comF) [Pseudomonas aeruginosa strain NY3045]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGATGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTATTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383