Detailed information NTG0411981

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: GKS41_RS02980
• Genome accession: NZ_CP047268
• Coordinates: 581058..582437 (+)
• Length: 459 a.a.
• NCBI ID: WP_014416742.1
• Uniprot ID: I2C0L1
• Organism: Bacillus velezensis strain DH8043
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 576058..587437

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKS41_RS02960	ctsR	576406..576870 (+)	465	WP_003156396.1	transcriptional regulator CtsR	-
GKS41_RS02965	-	576884..577441 (+)	558	WP_003156397.1	UvrB/UvrC motif-containing protein	-
GKS41_RS02970	-	577441..578532 (+)	1092	WP_159353704.1	protein arginine kinase	-
GKS41_RS02975	clpC	578529..580964 (+)	2436	WP_159353705.1	ATP-dependent protease ATP-binding subunit ClpC	Regulator
GKS41_RS02980	radA	581058..582437 (+)	1380	WP_014416742.1	DNA repair protein RadA	Machinery gene
GKS41_RS02985	disA	582441..583523 (+)	1083	WP_003156401.1	DNA integrity scanning diadenylate cyclase DisA	-
GKS41_RS02990	-	583636..584736 (+)	1101	WP_003156403.1	PIN/TRAM domain-containing protein	-
GKS41_RS02995	ispD	584749..585447 (+)	699	WP_003156405.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
GKS41_RS03000	ispF	585440..585916 (+)	477	WP_003156407.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-

Sequence

Protein

• Length: 459 a.a.
• Molecular weight: 49547.98 Da
• Isoelectric Point: 7.1760

>NTDB_id=411981 GKS41_RS02980 WP_014416742.1 581058..582437(+) (radA) [Bacillus velezensis strain DH8043]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide

• Length: 1380 bp

>NTDB_id=411981 GKS41_RS02980 WP_014416742.1 581058..582437(+) (radA) [Bacillus velezensis strain DH8043]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCACACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGCTAGTATTGATCGGCGGTGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACAGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATTGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GCTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGTATGGCTACAGGCATTGACCACAACAGGGTGTCGTTAATTATGGCAGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGACTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (364-430)

• AAA domain (AAA_25) (73-222)

3D structure

• AlphaFold DB: I2C0L1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	93.45	99.782	0.932
radA	Streptococcus pneumoniae Rx1	63.797	98.693	0.63
radA	Streptococcus pneumoniae D39	63.797	98.693	0.63
radA	Streptococcus pneumoniae R6	63.797	98.693	0.63
radA	Streptococcus pneumoniae TIGR4	63.797	98.693	0.63
radA	Streptococcus mitis NCTC 12261	63.797	98.693	0.63
radA	Streptococcus mitis SK321	63.576	98.693	0.627