Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   GRT08_RS12985 Genome accession   NZ_CP047234
Coordinates   2649861..2652221 (-) Length   786 a.a.
NCBI ID   WP_377802752.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2644861..2657221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS12965 (GRT08_12775) - 2644936..2646498 (+) 1563 WP_071391557.1 SH3 domain-containing protein -
  GRT08_RS12970 (GRT08_12780) dtd 2646525..2646968 (-) 444 WP_128574628.1 D-aminoacyl-tRNA deacylase -
  GRT08_RS12975 (GRT08_12785) relA 2646981..2649185 (-) 2205 WP_060674703.1 GTP diphosphokinase -
  GRT08_RS12980 (GRT08_12790) - 2649343..2649855 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  GRT08_RS12985 (GRT08_12795) recJ 2649861..2652221 (-) 2361 WP_377802752.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  GRT08_RS12990 (GRT08_12800) - 2652277..2652603 (-) 327 WP_043021678.1 lipopolysaccharide assembly LapA domain-containing protein -
  GRT08_RS12995 (GRT08_12805) - 2652667..2653164 (-) 498 WP_042635388.1 cation:proton antiporter regulatory subunit -
  GRT08_RS13000 (GRT08_12810) secDF 2653296..2655515 (-) 2220 WP_039254370.1 protein translocase subunit SecDF -
  GRT08_RS13005 (GRT08_12815) - 2655552..2655848 (-) 297 WP_007408189.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87414.93 Da        Isoelectric Point: 5.0300

>NTDB_id=411698 GRT08_RS12985 WP_377802752.1 2649861..2652221(-) (recJ) [Bacillus amyloliquefaciens strain FJ4]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVKSITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGIVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLICFNEKNAQKLREKELSGELAEITSESEAKALQLDGKYAVFLDVPPSADR
LQQLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=411698 GRT08_RS12985 WP_377802752.1 2649861..2652221(-) (recJ) [Bacillus amyloliquefaciens strain FJ4]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCACTCAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTCACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGCTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATCGAAAGGCTTCGGACGACAAATCGGCCGGGCCTCAAAGCCCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCGCCGAGGCTCAATGCAGTCGGAAGAATAGAACAGGCCGATC
CCGCCGTCCATCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGAGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGTCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGAATCGCTAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTGTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCAGGAATGACGTTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTAAAAGCATTACAGAATTAAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTACTTGATGATGTGCGCCTGATCGGAGCGAATAAAAACCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTTGAAGCCGGAATTGTCCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGACGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCCGGGGCAAGCGGACGTGGGAAGATACGGTCACCGCGCTGCCGAAGGAAAA
ACGGGTGCTCATCTGCTTTAACGAAAAAAACGCTCAGAAGCTCCGTGAAAAAGAGCTGTCGGGCGAGCTTGCCGAGATCA
CTTCGGAATCCGAGGCGAAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCAATTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCGCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAGGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHHA1 domain (DHHA1)

(315-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

82.061

100

0.821

Multiple sequence alignment