Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   H4J01_RS01530 Genome accession   NZ_CP059840
Coordinates   312902..313666 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain E2348/69     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 307902..318666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4J01_RS01505 yhhJ 309744..310868 (+) 1125 WP_001314210.1 ABC transporter permease -
  H4J01_RS01510 - 310941..311216 (+) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  H4J01_RS01515 - 311213..311572 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  H4J01_RS01520 nikR 311692..312093 (-) 402 WP_001190063.1 nickel-responsive transcriptional regulator NikR -
  H4J01_RS01525 nikE 312099..312905 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  H4J01_RS01530 amiE 312902..313666 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  H4J01_RS01535 nikC 313666..314499 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  H4J01_RS01540 nikB 314496..315440 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  H4J01_RS01545 nikA 315440..317014 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  H4J01_RS01550 acpT 317125..317712 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=411271 H4J01_RS01530 WP_001136232.1 312902..313666(-) (amiE) [Escherichia coli strain E2348/69]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=411271 H4J01_RS01530 WP_001136232.1 312902..313666(-) (amiE) [Escherichia coli strain E2348/69]
ATGCCGCAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCATGGCGTATCGTTAACCCTGCA
ACGTGGGCGCGTGCTGGCGTTAGTCGGCGGAAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGTATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTGCACACCATGCACACTCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398