Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GQR42_RS01875 Genome accession   NZ_CP046973
Coordinates   375766..376881 (+) Length   371 a.a.
NCBI ID   WP_158198683.1    Uniprot ID   -
Organism   Microcystis aeruginosa FD4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370766..381881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQR42_RS01855 (GQR42_01870) - 371407..371961 (+) 555 WP_158198679.1 YqeG family HAD IIIA-type phosphatase -
  GQR42_RS01860 (GQR42_01875) - 372550..373323 (+) 774 WP_158198680.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  GQR42_RS01865 (GQR42_01880) - 373364..374152 (+) 789 WP_158198681.1 ECF transporter S component -
  GQR42_RS01870 (GQR42_01885) ilvC 374271..375263 (-) 993 WP_158198682.1 ketol-acid reductoisomerase -
  GQR42_RS01875 (GQR42_01890) pilM 375766..376881 (+) 1116 WP_158198683.1 type IV pilus assembly protein PilM Machinery gene
  GQR42_RS29800 (GQR42_01895) - 376891..377660 (+) 770 Protein_357 PilN domain-containing protein -
  GQR42_RS01885 (GQR42_01900) - 377657..378448 (+) 792 WP_158198684.1 pilus assembly protein -
  GQR42_RS01890 (GQR42_01905) - 378520..380619 (+) 2100 WP_158198685.1 secretin N-terminal domain-containing protein -
  GQR42_RS01895 (GQR42_01910) - 381111..381803 (+) 693 Protein_360 IS1 family transposase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41081.03 Da        Isoelectric Point: 4.4263

>NTDB_id=409764 GQR42_RS01875 WP_158198683.1 375766..376881(+) (pilM) [Microcystis aeruginosa FD4]
MVSFFKKLLTKKNQGVGLEITPECLNVAQIAKQGQNYKLVKYCSSALPEGIFEEGKIVDSPALAELIQEVLKENKINSKR
VATGVPMRESIIRIIPIPSELDEQELRDLVLNHEASLYLPYPREEVDLDYQKLGYFQDEDGIEKVQVLLVATRREITDAY
RSTLQRAGLQVDVLEINSFALIRTIREQLRQFSSNEAAVIVDIEFDNTEIAIVVDGVPQFSRTVPIGTFQLQNALSRAMN
LPTSRSPDILLGMTIPVTAFDSLSTNTGTSGVSTNPGMTALMRVLGELTDELRRSINFYLNQSDDLEIVQLLLAGPGGGI
AQLDEYFTQRLSLPTMAIDPITSLNLDTTQEIPNTQRPGLGTVLGLGLREV

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=409764 GQR42_RS01875 WP_158198683.1 375766..376881(+) (pilM) [Microcystis aeruginosa FD4]
ATGGTAAGTTTTTTTAAGAAGCTATTGACAAAAAAAAATCAAGGTGTTGGGTTAGAGATTACCCCAGAGTGCCTTAACGT
CGCTCAGATCGCCAAACAGGGACAAAATTATAAATTAGTTAAATATTGTTCGTCGGCTCTCCCGGAAGGCATCTTTGAGG
AGGGAAAAATCGTCGATTCCCCCGCTTTAGCGGAATTGATCCAGGAAGTCCTCAAGGAAAATAAAATTAACAGCAAACGA
GTCGCTACTGGTGTCCCCATGCGCGAGTCAATTATCCGGATTATCCCCATTCCTTCGGAATTGGACGAGCAGGAATTGCG
AGATCTGGTCTTAAATCATGAGGCTAGTTTATACCTTCCCTATCCTCGCGAGGAGGTGGATCTCGACTACCAAAAACTGG
GCTACTTTCAGGATGAAGACGGGATTGAAAAGGTACAAGTCTTATTAGTTGCCACTCGTCGGGAGATTACCGATGCCTAT
AGGTCAACCCTCCAACGGGCAGGATTACAGGTGGATGTGCTGGAAATTAATAGTTTTGCCCTAATTCGGACGATTCGAGA
GCAGTTGCGACAATTTAGTTCCAATGAAGCCGCCGTTATTGTTGATATAGAATTTGATAACACGGAAATTGCCATCGTGG
TGGACGGCGTTCCCCAATTTTCCCGCACTGTCCCCATCGGCACCTTCCAGCTACAAAATGCCCTTTCCAGGGCGATGAAT
TTGCCCACCTCCCGCAGTCCTGATATTCTTTTGGGAATGACAATCCCGGTTACGGCCTTTGATAGTCTTAGCACCAATAC
CGGCACTTCCGGAGTCTCTACTAACCCTGGGATGACGGCACTGATGCGGGTTTTAGGGGAATTAACCGATGAATTGCGGC
GATCGATTAACTTCTATCTTAACCAAAGTGATGACCTGGAAATCGTCCAGTTACTTTTAGCTGGCCCCGGGGGCGGTATA
GCGCAACTAGACGAGTATTTTACTCAACGTCTCAGCCTCCCCACCATGGCAATCGATCCGATCACATCACTGAACTTAGA
TACAACTCAAGAGATTCCTAATACCCAAAGACCGGGTTTAGGAACAGTTCTCGGTTTAGGATTACGGGAGGTATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)

Type IV pilus assembly protein PilM; (PilM_2)

(287-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

75.135

99.73

0.749

Multiple sequence alignment