Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   GP482_RS01995 Genome accession   NZ_CP046875
Coordinates   396455..397321 (+) Length   288 a.a.
NCBI ID   WP_158913424.1    Uniprot ID   A0A6G8I1C3
Organism   Streptococcus ruminicola strain CNU_77-61     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 391455..402321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP482_RS01965 (GP482_01965) - 391685..392560 (+) 876 WP_158913422.1 F0F1 ATP synthase subunit gamma -
  GP482_RS01970 (GP482_01970) atpD 392628..394034 (+) 1407 WP_039697146.1 F0F1 ATP synthase subunit beta -
  GP482_RS01975 (GP482_01975) - 394047..394463 (+) 417 WP_004231089.1 F0F1 ATP synthase subunit epsilon -
  GP482_RS01980 (GP482_01980) - 394586..394822 (+) 237 WP_004231090.1 DUF1146 family protein -
  GP482_RS01985 (GP482_01985) murA 394884..396158 (+) 1275 WP_074563389.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  GP482_RS01990 (GP482_01990) - 396162..396350 (+) 189 WP_020916495.1 DNA-directed RNA polymerase subunit beta -
  GP482_RS01995 (GP482_01995) endA 396455..397321 (+) 867 WP_158913424.1 DNA/RNA non-specific endonuclease Machinery gene
  GP482_RS02000 (GP482_02000) pheS 397613..398656 (+) 1044 WP_014334511.1 phenylalanine--tRNA ligase subunit alpha -
  GP482_RS02005 (GP482_02005) - 398689..399213 (+) 525 WP_158913426.1 N-acetyltransferase -
  GP482_RS02010 (GP482_02010) pheT 399283..401688 (+) 2406 WP_158913428.1 phenylalanine--tRNA ligase subunit beta -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 30931.12 Da        Isoelectric Point: 8.9984

>NTDB_id=409106 GP482_RS01995 WP_158913424.1 396455..397321(+) (endA) [Streptococcus ruminicola strain CNU_77-61]
MAKKSKLSKQTKSFISLLVLLVSIGAGWLTTSDSSDPVNQVISLITGESSAKIVSNQSSANQATPSQELADSVLTDSVKA
QLGSNLEWNGAGAYLVNGNKNDLNARVASKPYANNKTKIVQGQTIPTVANALLSKSTRQYRSREETRNGSTSWTPAGWHQ
VQNLSGTYSHAVDRGHLLGYALVGGLKGFDASTSNPENVATQAAWANEASSEDSTGQNYYEGQVRRALDQNKRVRYRVTL
IYDGDNILASGTHLEAKSSDGSLEFNVFIPNVQSGLSFDYYSGQVSVN

Nucleotide


Download         Length: 867 bp        

>NTDB_id=409106 GP482_RS01995 WP_158913424.1 396455..397321(+) (endA) [Streptococcus ruminicola strain CNU_77-61]
ATGGCAAAGAAATCAAAACTGTCTAAACAGACAAAATCATTTATTTCTTTATTAGTTTTATTGGTTAGTATTGGTGCGGG
TTGGCTGACAACTTCAGATTCAAGTGATCCTGTTAATCAAGTTATTTCCTTGATTACGGGAGAATCATCTGCAAAGATTG
TGTCAAATCAGTCTTCTGCTAATCAAGCAACACCAAGTCAAGAATTAGCAGACTCAGTCTTAACAGACAGTGTTAAAGCT
CAGCTTGGTTCAAATTTAGAATGGAACGGTGCGGGAGCTTATTTGGTTAACGGAAATAAAAACGATTTGAATGCAAGAGT
AGCCAGCAAACCTTATGCCAATAACAAAACAAAAATTGTGCAAGGTCAGACTATTCCAACGGTCGCAAATGCTTTGTTGA
GTAAGTCTACACGTCAATATAGAAGCCGTGAGGAAACTAGAAATGGCTCAACTTCATGGACACCAGCGGGCTGGCACCAA
GTGCAAAATCTTTCTGGAACATACAGTCACGCCGTTGACCGTGGGCACTTGCTCGGCTATGCTTTAGTTGGTGGCTTAAA
AGGCTTTGATGCTTCAACAAGCAATCCTGAAAATGTAGCCACTCAGGCAGCCTGGGCAAATGAAGCTAGTTCAGAAGACT
CAACGGGTCAAAATTACTACGAAGGTCAGGTCAGAAGAGCACTTGATCAGAATAAACGTGTTCGTTACCGCGTGACTTTA
ATCTATGATGGCGATAATATTCTTGCTAGCGGGACACATTTGGAAGCAAAATCATCTGACGGTAGTTTAGAGTTTAATGT
TTTCATTCCGAATGTCCAAAGTGGTTTAAGCTTTGATTATTATTCAGGTCAGGTTAGTGTGAATTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(106-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8I1C3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

67.797

81.944

0.556
  endA Streptococcus pneumoniae D39

67.797

81.944

0.556
  endA Streptococcus pneumoniae R6

67.797

81.944

0.556
  endA Streptococcus pneumoniae TIGR4

67.797

81.944

0.556

Multiple sequence alignment