Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HXS77_RS01300 Genome accession   NZ_CP058355
Coordinates   293021..293785 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain AMSCJX03     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 288021..298785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXS77_RS01290 (HXS77_01290) nikR 291811..292212 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HXS77_RS01295 (HXS77_01295) nikE 292218..293024 (-) 807 WP_178887046.1 nickel import ATP-binding protein NikE -
  HXS77_RS01300 (HXS77_01300) amiE 293021..293785 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  HXS77_RS01305 (HXS77_01305) nikC 293785..294618 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  HXS77_RS01310 (HXS77_01310) nikB 294615..295559 (-) 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -
  HXS77_RS01315 (HXS77_01315) nikA 295559..297133 (-) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  HXS77_RS01320 (HXS77_01320) acpT 297244..297831 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=405377 HXS77_RS01300 WP_001136229.1 293021..293785(-) (amiE) [Escherichia coli strain AMSCJX03]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=405377 HXS77_RS01300 WP_001136229.1 293021..293785(-) (amiE) [Escherichia coli strain AMSCJX03]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment