Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HWH78_RS00715 Genome accession   NZ_CP056774
Coordinates   156202..156627 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain CDN129     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 151202..161627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWH78_RS00695 (HWH78_00695) ileS 151359..154190 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  HWH78_RS00700 (HWH78_00700) lspA 154183..154692 (+) 510 WP_003102615.1 signal peptidase II -
  HWH78_RS00705 (HWH78_00705) fkpB 154685..155125 (+) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HWH78_RS00710 (HWH78_00710) ispH 155211..156155 (+) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HWH78_RS00715 (HWH78_00715) comF 156202..156627 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HWH78_RS00720 (HWH78_00720) pilY2 156624..156971 (-) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  HWH78_RS00725 (HWH78_00725) pilY1 156973..160464 (-) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  HWH78_RS00730 (HWH78_00730) pilX 160476..161063 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=403467 HWH78_RS00715 WP_003094721.1 156202..156627(-) (comF) [Pseudomonas aeruginosa strain CDN129]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=403467 HWH78_RS00715 WP_003094721.1 156202..156627(-) (comF) [Pseudomonas aeruginosa strain CDN129]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment