Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HED12_RS11595 Genome accession   NZ_CP056077
Coordinates   2325579..2326343 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain MS1170     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2320579..2331343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HED12_RS11570 (HED12_11555) yhhJ 2322421..2323545 (+) 1125 WP_001314210.1 ABC transporter permease -
  HED12_RS11575 (HED12_11560) - 2323618..2323893 (+) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  HED12_RS11580 (HED12_11565) - 2323890..2324249 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  HED12_RS11585 (HED12_11570) nikR 2324369..2324770 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HED12_RS11590 (HED12_11575) nikE 2324776..2325582 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  HED12_RS11595 (HED12_11580) amiE 2325579..2326343 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  HED12_RS11600 (HED12_11585) nikC 2326343..2327176 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  HED12_RS11605 (HED12_11590) nikB 2327173..2328117 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  HED12_RS11610 (HED12_11595) nikA 2328117..2329691 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  HED12_RS11615 (HED12_11600) acpT 2329802..2330389 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=403340 HED12_RS11595 WP_001136232.1 2325579..2326343(-) (amiE) [Escherichia coli strain MS1170]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=403340 HED12_RS11595 WP_001136232.1 2325579..2326343(-) (amiE) [Escherichia coli strain MS1170]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398