Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   GL184_RS01540 Genome accession   NZ_CP046356
Coordinates   289277..290101 (+) Length   274 a.a.
NCBI ID   WP_001036786.1    Uniprot ID   A0A0B7LDF1
Organism   Streptococcus pneumoniae strain 521     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 284277..295101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL184_RS01505 (GL184_01530) asnA 284367..285359 (+) 993 WP_000747993.1 aspartate--ammonia ligase -
  GL184_RS01510 (GL184_01535) rsmD 285424..285963 (+) 540 WP_000706962.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GL184_RS01515 (GL184_01540) coaD 285953..286441 (+) 489 WP_001280742.1 pantetheine-phosphate adenylyltransferase -
  GL184_RS01520 (GL184_01545) sepM 286425..287462 (+) 1038 WP_000727276.1 SepM family pheromone-processing serine protease Regulator
  GL184_RS01525 (GL184_01550) - 287520..287750 (+) 231 WP_000250392.1 DUF1146 family protein -
  GL184_RS01530 (GL184_01555) murA 287774..289057 (+) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  GL184_RS01535 (GL184_01560) - 289050..289238 (+) 189 WP_001813390.1 DNA-directed RNA polymerase subunit beta -
  GL184_RS01540 (GL184_01565) endA 289277..290101 (+) 825 WP_001036786.1 DNA-entry nuclease EndA Machinery gene
  GL184_RS01545 (GL184_01570) - 290392..291723 (+) 1332 WP_016398203.1 hemolysin family protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29921.59 Da        Isoelectric Point: 10.1197

>NTDB_id=403097 GL184_RS01540 WP_001036786.1 289277..290101(+) (endA) [Streptococcus pneumoniae strain 521]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=403097 GL184_RS01540 WP_001036786.1 289277..290101(+) (endA) [Streptococcus pneumoniae strain 521]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LDF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.635

100

0.996
  endA Streptococcus pneumoniae D39

99.635

100

0.996
  endA Streptococcus pneumoniae R6

99.635

100

0.996
  endA Streptococcus pneumoniae TIGR4

99.635

100

0.996

Multiple sequence alignment