Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   GMB29_RS20240 Genome accession   NZ_CP046266
Coordinates   4199705..4202062 (-) Length   785 a.a.
NCBI ID   WP_136351904.1    Uniprot ID   A0A4S4C3S5
Organism   Metabacillus sediminilitoris strain DSL-17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4194705..4207062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMB29_RS20225 (GMB29_20225) dtd 4196177..4196617 (-) 441 WP_136351901.1 D-aminoacyl-tRNA deacylase -
  GMB29_RS20230 (GMB29_20230) - 4196631..4198838 (-) 2208 WP_136351902.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  GMB29_RS20235 (GMB29_20235) - 4199176..4199688 (-) 513 WP_136351903.1 adenine phosphoribosyltransferase -
  GMB29_RS20240 (GMB29_20240) recJ 4199705..4202062 (-) 2358 WP_136351904.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  GMB29_RS20245 (GMB29_20245) - 4202407..4202700 (-) 294 WP_136351905.1 post-transcriptional regulator -
  GMB29_RS20250 (GMB29_20250) spoVB 4202858..4204429 (+) 1572 WP_136351906.1 stage V sporulation protein B -
  GMB29_RS20255 (GMB29_20255) - 4204524..4205180 (-) 657 WP_136351907.1 DUF421 domain-containing protein -
  GMB29_RS20260 (GMB29_20260) - 4205406..4205792 (+) 387 WP_136351908.1 TIGR04086 family membrane protein -
  GMB29_RS20265 (GMB29_20265) yajC 4205839..4206099 (-) 261 WP_136351909.1 preprotein translocase subunit YajC -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 88221.07 Da        Isoelectric Point: 5.1981

>NTDB_id=402098 GMB29_RS20240 WP_136351904.1 4199705..4202062(-) (recJ) [Metabacillus sediminilitoris strain DSL-17]
MLKPKTRWVVQTSDESLINTLIDELSITRLVASLLVNRGITTTQEAREFLQAEQKAFHDPFLLKDMDKAVRRIMKAIEAQ
EKILIYGDYDADGVSSTTVLLTTLRKLGAIADFYIPNRFTEGYGPNETAFRQAHDNGFTLIITVDTGIAAVHEADIAREL
GVDLIITDHHEPGPTLPSALAIIHPKQPQCSYPFKDLAGVGVAFKLSHALLGELPSDLLEVAAIGTIADLVPLHGENRLI
AKKGINQLKTTKRIGLQALLKIAKVNSSEINEDTIGFALAPRINAVGRLQSADPAVDLLLSDDSEEAFDIAQEIDSLNKE
RQKIVSSMTDEAIEEVETNFPIAENPVLVIAREGWNPGVVGIVASRLVERFYRPTIVLSIDKEKGIAKGSARSIVGFDLF
ENLSTCRDILPHFGGHPMAAGMTLELENVDLLRTRLVKNAESILSDDDFTPITKVDISCNLEEITLKAIEEMQQLAPFGM
YNPKPVIKVENVTLANVRKIGAEQNHLKLVFEQEEHQLDSVGFGLGHIHDELSPAVTLSAIGELSINEWNNFRKPQLMLQ
DVKVDSWQLFDFRGTRNVEKLLKTVALNENTLIITYRFSIFEKLKKQGFQKNTVFVDSMSNAQQMDVKNKNLVLLDIPSS
LDLLESLFTHGKPDRIYSVLQQEADHFFSTIPTREHFKWYYGFLLKKGPFKLEEHGEQLAMHKGWSKETIEFMSQVFFDL
EFVTIENGVITTKSSSQKRDLAESKTYAQKQMQIELEKTLLYTSYMQLKEWFEERFTATNPHVFV

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=402098 GMB29_RS20240 WP_136351904.1 4199705..4202062(-) (recJ) [Metabacillus sediminilitoris strain DSL-17]
ATGTTAAAACCTAAAACCAGATGGGTCGTACAAACATCTGATGAATCTTTAATTAATACACTAATAGATGAATTGTCGAT
TACACGTCTAGTTGCATCACTACTCGTTAATCGCGGCATTACAACAACTCAAGAAGCTCGTGAATTTTTACAAGCGGAAC
AAAAGGCTTTTCACGATCCTTTTTTATTAAAAGATATGGACAAGGCTGTACGCCGAATAATGAAAGCGATCGAAGCCCAA
GAAAAAATATTAATCTATGGTGACTATGATGCAGACGGTGTGAGTAGCACAACAGTTCTGTTAACTACTTTAAGAAAATT
AGGAGCAATAGCTGATTTTTATATACCTAATCGATTTACTGAAGGATACGGGCCAAATGAAACAGCATTTAGACAAGCAC
ATGATAACGGGTTTACCCTTATCATCACAGTCGATACAGGTATTGCAGCCGTACATGAGGCTGATATCGCAAGAGAGCTT
GGTGTGGATCTTATTATTACTGATCACCATGAACCAGGCCCTACTTTACCTTCTGCGTTAGCTATAATTCATCCGAAGCA
ACCACAATGTTCATACCCTTTTAAGGATTTAGCTGGTGTTGGAGTTGCTTTCAAATTAAGCCACGCCCTTCTCGGCGAAC
TGCCATCAGATTTATTAGAAGTTGCAGCGATAGGAACGATTGCAGATCTTGTCCCACTTCATGGTGAAAATAGACTTATC
GCCAAAAAGGGTATTAATCAATTGAAAACAACCAAACGCATAGGATTACAAGCATTATTAAAAATTGCAAAAGTTAATAG
CTCAGAGATTAACGAAGATACAATTGGCTTTGCCTTAGCGCCTCGTATTAATGCGGTCGGCCGTCTCCAGTCTGCAGATC
CGGCAGTTGACTTATTATTATCAGATGACTCTGAAGAAGCTTTTGATATTGCACAAGAAATAGATTCATTAAATAAAGAA
CGTCAAAAAATTGTAAGCTCTATGACAGATGAAGCAATTGAGGAAGTAGAAACAAATTTTCCAATTGCCGAAAATCCAGT
TCTTGTTATTGCTAGGGAAGGGTGGAATCCAGGAGTTGTCGGGATTGTCGCATCACGATTAGTTGAACGCTTTTACAGAC
CAACAATTGTCCTTAGTATTGATAAAGAAAAAGGTATTGCAAAAGGTTCCGCAAGAAGTATTGTAGGATTTGATTTATTT
GAAAATCTCTCAACCTGCCGAGATATTTTACCGCATTTTGGTGGACACCCAATGGCAGCAGGGATGACACTTGAATTGGA
AAATGTAGACTTATTAAGAACACGTTTAGTGAAAAATGCAGAGAGTATTTTATCAGATGATGACTTTACACCAATTACAA
AGGTCGATATAAGCTGTAATCTGGAGGAAATTACCTTAAAAGCTATTGAGGAAATGCAACAGCTCGCACCTTTTGGGATG
TACAATCCAAAACCTGTTATTAAAGTCGAGAATGTAACATTAGCGAATGTGCGCAAAATTGGGGCAGAACAAAATCATCT
AAAGCTCGTCTTTGAGCAAGAAGAACATCAATTAGATAGTGTTGGCTTTGGATTAGGACATATTCATGATGAATTATCAC
CAGCTGTTACCTTATCTGCAATTGGGGAATTATCGATCAACGAATGGAATAACTTTAGAAAACCGCAATTAATGCTTCAG
GATGTAAAGGTAGATAGCTGGCAGCTGTTTGACTTCAGAGGAACTCGAAATGTTGAAAAATTATTAAAAACAGTTGCATT
AAACGAAAACACTTTGATTATTACGTATAGATTTTCTATATTTGAGAAATTGAAAAAACAAGGTTTTCAAAAGAATACGG
TCTTTGTTGACTCAATGTCTAATGCTCAGCAAATGGACGTGAAAAATAAAAATCTTGTCCTATTAGATATTCCTTCTTCA
CTTGATCTACTTGAATCGTTATTTACTCATGGAAAACCTGATAGAATATATTCTGTGCTCCAACAAGAAGCAGATCACTT
TTTTTCGACAATTCCAACAAGAGAACATTTTAAATGGTATTATGGTTTTCTCTTAAAAAAAGGCCCATTTAAGCTAGAAG
AACATGGTGAGCAACTTGCAATGCATAAAGGATGGTCAAAAGAAACAATTGAATTTATGTCACAGGTGTTTTTTGATCTA
GAATTTGTTACAATAGAGAATGGTGTTATTACGACAAAATCTTCGTCTCAAAAAAGAGACCTGGCAGAATCGAAAACATA
TGCTCAAAAGCAAATGCAAATCGAACTAGAAAAAACGTTATTATATACATCTTACATGCAGCTTAAAGAATGGTTTGAAG
AAAGATTTACAGCTACGAATCCGCATGTATTTGTATAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-772)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4S4C3S5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

57.124

98.344

0.562

Multiple sequence alignment