Detailed information NTG0401709

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GFS31_RS14310
• Genome accession: NZ_CP046155
• Coordinates: 3232837..3233943 (-)
• Length: 368 a.a.
• NCBI ID: WP_198805453.1
• Uniprot ID: A0A7T5E5E7
• Organism: Leptolyngbya sp. BL0902
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3227837..3238943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFS31_RS14285	-	3228250..3228474 (+)	225	WP_198808226.1	DUF433 domain-containing protein	-
GFS31_RS14290 (GFS31_30280)	-	3228471..3228815 (+)	345	WP_198805448.1	DUF5615 family PIN-like protein	-
GFS31_RS14295 (GFS31_30290)	-	3228907..3231033 (-)	2127	WP_225907431.1	AMIN domain-containing protein	-
GFS31_RS14300 (GFS31_30300)	-	3231153..3232052 (-)	900	WP_198805450.1	hypothetical protein	-
GFS31_RS14305 (GFS31_30310)	-	3232052..3232834 (-)	783	WP_198805452.1	PilN domain-containing protein	-
GFS31_RS14310 (GFS31_30320)	pilM	3232837..3233943 (-)	1107	WP_198805453.1	type IV pilus assembly protein PilM	Machinery gene
GFS31_RS14315 (GFS31_30340)	purT	3234404..3235603 (+)	1200	WP_198805454.1	formate-dependent phosphoribosylglycinamide formyltransferase	-
GFS31_RS14320 (GFS31_30350)	-	3235753..3236277 (-)	525	WP_198805456.1	hypothetical protein	-
GFS31_RS14325 (GFS31_30360)	-	3236505..3237278 (-)	774	WP_198805457.1	hypothetical protein	-
GFS31_RS14330 (GFS31_30370)	-	3237970..3238494 (-)	525	WP_198805458.1	hypothetical protein	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 39961.75 Da
• Isoelectric Point: 4.2580

>NTDB_id=401709 GFS31_RS14310 WP_198805453.1 3232837..3233943(-) (pilM) [Leptolyngbya sp. BL0902]
MDSLKALFSRKPRGVGIELAPDKVTLAQLKKQGNSLKLAALESVELPEGVYEEGQIIDSAAMADAIQALLTQSKVKVKRA
TTAIPGRAIAKIIPVPSELDDEELREMVLNQEASLYLPFPREEADVDYQKLGYFVDEDGIEKVKVLLVGVRKDLTDPYID
VFAQAGLGLNVLETSSFALIRTIREQLRQFTPQEAAAVVDIEFESTEISIVVDGVPHFSRTVPTGTFQIQSALSRAMNLP
PSRNTELLQGMTVPVQSMDTVGAMQVGGGTNPGTTAMLRVLGELSDELRRSIDFYLNQEEDMEVAQLLLAGPGASIGQLD
EFLAQRLSIPTSQIDPISALSLELTEDIPESQRAGLATVIGLGLREVM

Nucleotide

• Length: 1107 bp

>NTDB_id=401709 GFS31_RS14310 WP_198805453.1 3232837..3233943(-) (pilM) [Leptolyngbya sp. BL0902]
GTGGATAGTTTAAAAGCTCTTTTTAGTCGCAAACCTCGGGGTGTGGGTATCGAACTGGCCCCCGATAAAGTCACCCTGGC
CCAGCTCAAGAAACAGGGCAACAGCCTGAAGCTGGCCGCCCTAGAGTCGGTGGAGTTGCCGGAGGGGGTCTACGAGGAAG
GTCAAATCATTGACTCCGCCGCCATGGCCGACGCCATTCAGGCTTTGCTGACCCAGAGCAAGGTGAAGGTGAAGCGGGCC
ACCACAGCCATTCCTGGGCGGGCCATTGCCAAGATTATCCCGGTGCCCTCGGAGTTGGATGACGAGGAACTGCGGGAGAT
GGTGCTGAACCAGGAGGCCAGCCTCTACCTGCCCTTCCCCCGCGAAGAGGCCGACGTGGACTACCAAAAGCTGGGCTACT
TTGTGGATGAGGACGGCATCGAGAAGGTGAAGGTGCTGCTGGTGGGGGTACGCAAGGATTTGACCGACCCCTACATTGAT
GTGTTTGCCCAAGCAGGGCTGGGGCTGAATGTGCTAGAAACCTCTAGCTTTGCCCTGATTCGCACCATTCGCGAACAGCT
ACGGCAGTTCACACCCCAGGAAGCCGCCGCCGTGGTGGATATTGAGTTTGAAAGCACCGAAATTTCCATTGTGGTGGATG
GGGTGCCCCACTTTTCCCGCACGGTGCCCACCGGAACATTTCAGATCCAAAGCGCCCTCAGTCGGGCCATGAACCTGCCT
CCCTCCCGCAATACGGAGCTGCTTCAGGGGATGACCGTCCCCGTTCAGAGTATGGATACGGTGGGGGCGATGCAGGTGGG
GGGCGGCACCAATCCGGGGACGACCGCCATGCTGCGGGTGCTGGGCGAGTTATCCGACGAACTGCGTCGATCCATCGACT
TCTACCTCAACCAAGAGGAAGACATGGAGGTGGCCCAGTTATTGCTGGCGGGGCCGGGGGCCTCCATTGGCCAACTGGAT
GAGTTCTTGGCCCAGCGGTTGAGCATTCCCACCAGCCAGATTGACCCGATTTCGGCCCTGTCCTTAGAGCTAACCGAGGA
CATCCCCGAAAGTCAGCGGGCGGGGCTGGCCACGGTGATTGGGTTGGGCCTACGGGAGGTGATGTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (283-365)

• Type IV pilus assembly protein PilM; (PilM_2) (16-239)

3D structure

• AlphaFold DB: A0A7T5E5E7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Synechocystis sp. PCC 6803	60.44	98.913	0.598