Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HUK78_RS25225 Genome accession   NZ_CP054794
Coordinates   5384386..5384811 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain A0002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5379386..5389811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK78_RS25210 (HUK78_25205) pilX 5379956..5380543 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  HUK78_RS25215 (HUK78_25210) pilY1 5380555..5384040 (+) 3486 WP_023518093.1 type 4a pilus biogenesis protein PilY1 -
  HUK78_RS25220 (HUK78_25215) pilY2 5384042..5384389 (+) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  HUK78_RS25225 (HUK78_25220) comF 5384386..5384811 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HUK78_RS25230 (HUK78_25225) ispH 5384858..5385802 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HUK78_RS25235 (HUK78_25230) fkpB 5385888..5386328 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HUK78_RS25240 (HUK78_25235) lspA 5386321..5386830 (-) 510 WP_003094728.1 signal peptidase II -
  HUK78_RS25245 (HUK78_25240) ileS 5386823..5389654 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=401569 HUK78_RS25225 WP_003094721.1 5384386..5384811(+) (comF) [Pseudomonas aeruginosa strain A0002]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=401569 HUK78_RS25225 WP_003094721.1 5384386..5384811(+) (comF) [Pseudomonas aeruginosa strain A0002]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383