Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GKC13_RS02315 Genome accession   NZ_CP046134
Coordinates   431862..432557 (+) Length   231 a.a.
NCBI ID   WP_014608614.1    Uniprot ID   A0AAU9H5B0
Organism   Streptococcus thermophilus strain MAG_rmk202_sterm     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426862..437557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC13_RS02295 (GKC13_02305) - 428921..429190 (-) 270 WP_011226404.1 GIY-YIG nuclease family protein -
  GKC13_RS02300 (GKC13_02310) - 429194..429958 (-) 765 WP_014608617.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  GKC13_RS09870 - 430008..430157 (-) 150 WP_014608616.1 polysaccharide deacetylase -
  GKC13_RS09875 (GKC13_02320) - 430266..430799 (-) 534 WP_014608615.1 polysaccharide deacetylase family protein -
  GKC13_RS02310 (GKC13_02325) - 430999..431754 (+) 756 WP_179970604.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  GKC13_RS02315 (GKC13_02330) comEA 431862..432557 (+) 696 WP_014608614.1 helix-hairpin-helix domain-containing protein Machinery gene
  GKC13_RS02320 (GKC13_02335) - 432547..434787 (+) 2241 WP_180479499.1 DNA internalization-related competence protein ComEC/Rec2 -
  GKC13_RS02325 (GKC13_02340) - 434873..436135 (+) 1263 WP_014608612.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  GKC13_RS02330 (GKC13_02345) - 436201..436746 (+) 546 WP_014608611.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 24602.52 Da        Isoelectric Point: 4.5661

>NTDB_id=401505 GKC13_RS02315 WP_014608614.1 431862..432557(+) (comEA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MKEKILAYVKDNRLFVSVIAVLMVIFCFFLWMTCGAGNSMEAETSYTDVTALSTSSSKQSSQSLSEASSQSKTEGSEKVK
SKVTVDVKGAVVNPGVYTLKEGARVTDVIQEAGGMTEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQTSTGQVSDKG
GQAVSKDGKINLNTATSEQLQTISGIGAKRTEDIIAYRESHGGFQSVDDLKNVSGIGDKTLDKIRESLYVA

Nucleotide


Download         Length: 696 bp        

>NTDB_id=401505 GKC13_RS02315 WP_014608614.1 431862..432557(+) (comEA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
GTGAAGGAAAAGATTCTAGCCTATGTCAAAGATAATCGTCTGTTTGTGAGTGTCATCGCTGTACTGATGGTGATTTTTTG
CTTCTTTTTATGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCGGAGACGTCTTATACAGATGTGACAGCTTTGTCAA
CGTCTTCATCCAAACAAAGTTCACAGTCTCTTTCTGAGGCGTCTTCCCAGTCAAAGACCGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAATCCAGGTGTCTATACGCTAAAGGAAGGCGCTAGGGTGACAGA
TGTCATTCAGGAAGCTGGAGGAATGACAGAAGATGCAGACGCTAAGAGTGTTAACTTAGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGATGAAAATGTTTCTGTCCTTGATCAAACAAGTACTGGTCAGGTCTCTGACAAAGGA
GGGCAGGCTGTATCTAAGGATGGTAAAATTAACTTAAATACAGCAACCTCAGAGCAGTTGCAAACCATTTCTGGAATTGG
AGCTAAGCGGACAGAGGATATCATTGCCTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGATGACTTGAAAAATGTCT
CAGGAATTGGTGATAAAACATTAGATAAAATCAGAGAGTCCCTCTATGTGGCTTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(167-228)

SLBB domain (SLBB)

(85-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

98.268

100

0.983
  comEA/celA/cilE Streptococcus mitis SK321

40.175

99.134

0.398
  comEA/celA/cilE Streptococcus pneumoniae D39

38.596

98.701

0.381
  comEA/celA/cilE Streptococcus pneumoniae R6

38.596

98.701

0.381
  comEA/celA/cilE Streptococcus pneumoniae Rx1

38.596

98.701

0.381
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

38.596

98.701

0.381
  comEA/celA/cilE Streptococcus mitis NCTC 12261

37.281

98.701

0.368

Multiple sequence alignment