Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   GKC27_RS12545 Genome accession   NZ_CP046130
Coordinates   2452844..2455168 (-) Length   774 a.a.
NCBI ID   WP_003216405.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2447844..2460168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS12530 (GKC27_12375) dtd 2449493..2449936 (-) 444 WP_003216527.1 D-aminoacyl-tRNA deacylase -
  GKC27_RS12535 (GKC27_12380) - 2449955..2452165 (-) 2211 WP_003216285.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  GKC27_RS12540 (GKC27_12385) - 2452308..2452820 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  GKC27_RS12545 (GKC27_12390) recJ 2452844..2455168 (-) 2325 WP_003216405.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  GKC27_RS12550 (GKC27_12395) - 2455250..2455603 (-) 354 WP_003216178.1 lipopolysaccharide assembly LapA domain-containing protein -
  GKC27_RS12555 (GKC27_12400) - 2455706..2456193 (-) 488 Protein_2432 cation:proton antiporter regulatory subunit -
  GKC27_RS12560 (GKC27_12405) secDF 2456372..2458600 (-) 2229 WP_095117848.1 protein translocase subunit SecDF -
  GKC27_RS12565 (GKC27_12410) - 2458642..2458938 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87374.02 Da        Isoelectric Point: 4.8943

>NTDB_id=401452 GKC27_RS12545 WP_003216405.1 2452844..2455168(-) (recJ) [Bacillus pumilus strain DSM 27]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPTQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDVRLEEIRTIGANQNHIKMSLKDEDQLLDCVGFHQGQLKEEVVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVKIPIHHVHAENVSTFDVEGKYVVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSIMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=401452 GKC27_RS12545 WP_003216405.1 2452844..2455168(-) (recJ) [Bacillus pumilus strain DSM 27]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTTGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAGGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTCCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACCGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAACTGCCTGAGGCACTTGCGATTGTTCATCCTAAGCA
GCCAGGCTGTGAGTATCCCTTTAAAGAACTTGCCGGTGTAGGCGTAGCATTTAAAGTGGCGCATGCACTTCTTGGAAAGC
TGCCAACTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTAGGCAGTCGAATAGACCAGGGCTGAAGGCGTTATTGAAAGAAGCAGGTGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCCGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCAATCGT
AGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGTGACATTCTGCCGCATTTTGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTTG
ATGCTGTATGCAAGGTAGACGAGTTGACGGTTGAAGCAATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGTGAGACTTGAAGAAATCCGAACAATTGGAGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGACGAAGACCAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAGTTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCACAGCTCATGCTAAAAGATGCC
CGAGTAGATGAGTGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGACCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAAAATCCCGATTCATCATGTCCATGCAGAAAATGTTT
CAACATTCGATGTAGAAGGCAAGTATGTTGTCTTTGCAGATGTTCCAGCAGATGAACACTTACTAAGGCAGCTGTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAGCGAGGAAGTTTCCCTCTCAAGGAGCAAGGAATGGAGCTTGCGAAACATA
GAGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACAGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATAATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHHA1 domain (DHHA1)

(309-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.053

100

0.673

Multiple sequence alignment