Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   GKC25_RS11835 Genome accession   NZ_CP046128
Coordinates   2329575..2331899 (-) Length   774 a.a.
NCBI ID   WP_342689802.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2324575..2336899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS11820 (GKC25_11700) dtd 2326224..2326667 (-) 444 WP_060596866.1 D-aminoacyl-tRNA deacylase -
  GKC25_RS11825 (GKC25_11705) - 2326686..2328896 (-) 2211 WP_268495743.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  GKC25_RS11830 (GKC25_11710) - 2329039..2329551 (-) 513 WP_034661543.1 adenine phosphoribosyltransferase -
  GKC25_RS11835 (GKC25_11715) recJ 2329575..2331899 (-) 2325 WP_342689802.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  GKC25_RS11840 (GKC25_11720) - 2331981..2332334 (-) 354 WP_095285506.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  GKC25_RS11845 (GKC25_11725) - 2332436..2332924 (-) 489 WP_034661552.1 cation:proton antiporter regulatory subunit -
  GKC25_RS11850 (GKC25_11730) secDF 2333111..2335339 (-) 2229 WP_034661556.1 protein translocase subunit SecDF -
  GKC25_RS11855 (GKC25_11735) - 2335381..2335677 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87452.08 Da        Isoelectric Point: 4.9269

>NTDB_id=401392 GKC25_RS11835 WP_342689802.1 2329575..2331899(-) (recJ) [Bacillus pumilus strain V17C11]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPAQLLDLAAIGTIADLVPLHDENRWL
TKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDRFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDARLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEIVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVNIPIYHVHAENVSTFDVEGKYIVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=401392 GKC25_RS11835 WP_342689802.1 2329575..2331899(-) (recJ) [Bacillus pumilus strain V17C11]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAATAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGCG
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTACGGGCCTAATGAACAGGCTTTCCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACGGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCCTTTAAAGAACTTGCCGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTCCTTGGAAAGC
TGCCAGCTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
ACCAAAAAAGGGCTTGTGCAGCTTAGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCTACTCT
TGAAGAAGCAAATGAAGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAGCAGGCAGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAAGAACTCAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATAGATTTTCACGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGCGACATTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTAAGAGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTCG
ATGCCGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGCGAGGCTTGAAGAGATCCGAACGATTGGGGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAATTGTCACAGGTA
GCCGCATTTCCGTAATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCTCAGCTTATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAATATCCCAATTTATCATGTCCATGCAGAAAATGTTT
CAACGTTCGATGTAGAGGGCAAGTATATCGTCTTTGCGGATGTTCCTGCAGATGAGCATTTGCTAAGGCAGCTTTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCTTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCCCTCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(309-437)

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676

Multiple sequence alignment