Detailed information NTG0400330

Bioinformatically predicted

Overview

• Name: pilH
• Type: Machinery gene
• Locus tag: GJV52_RS10235
• Genome accession: NZ_CP046027
• Coordinates: 2052603..2053268 (-)
• Length: 221 a.a.
• NCBI ID: WP_095503772.1
• Uniprot ID: -
• Organism: Neisseria brasiliensis strain N.177.16
• Function: type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	2045333..2054834	2052603..2053268	within	0

Gene organization within MGE regions

Location: 2045333..2054834

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJV52_RS10185 (GJV52_10185)	tnpA	2045333..2045749 (-)	417	WP_095503171.1	IS200/IS605 family transposase	-
GJV52_RS10190 (GJV52_10190)	-	2045794..2046936 (+)	1143	WP_095503172.1	RNA-guided endonuclease TnpB family protein	-
GJV52_RS10195 (GJV52_10195)	-	2047244..2048233 (+)	990	WP_095503778.1	ribose-phosphate pyrophosphokinase	-
GJV52_RS10200 (GJV52_10200)	-	2048301..2048873 (+)	573	WP_100564612.1	50S ribosomal protein L25/general stress protein Ctc	-
GJV52_RS10205 (GJV52_10205)	-	2049092..2049553 (-)	462	WP_195690038.1	PilX family type IV pilin	-
GJV52_RS10210 (GJV52_10210)	pilK	2049555..2050127 (-)	573	WP_095503774.1	PilX N-terminal domain-containing pilus assembly protein	Machinery gene
GJV52_RS10215 (GJV52_10215)	-	2050106..2050384 (-)	279	WP_154212894.1	hypothetical protein	-
GJV52_RS10220 (GJV52_10220)	-	2050445..2051251 (+)	807	WP_154212829.1	integrase core domain-containing protein	-
GJV52_RS13525	-	2051879..2051953 (-)	75	Protein_1979	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GJV52_RS10230 (GJV52_10230)	pilV	2051956..2052600 (-)	645	WP_229436967.1	type IV pilus modification protein PilV	-
GJV52_RS10235 (GJV52_10235)	pilH	2052603..2053268 (-)	666	WP_095503772.1	GspH/FimT family pseudopilin	Machinery gene
GJV52_RS10240 (GJV52_10240)	dnaB	2053428..2054834 (-)	1407	WP_095503771.1	replicative DNA helicase	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 24581.92 Da
• Isoelectric Point: 9.8796

>NTDB_id=400330 GJV52_RS10235 WP_095503772.1 2052603..2053268(-) (pilH) [Neisseria brasiliensis strain N.177.16]
MHAKQKGFTLIELLVVIAIIAIMAVIALPNMSQWIASRRVASNAEQIANLLRFARSEAVRLNIPVYVCPVQIKKDGKSNG
SCNASFGNQGLMAFADTDKNKQYQRTSTDIQLRAIILNSKDNDRITHSYYAYPFSGSKTTDSNVRWTFLPNGNFVRWNKS
PTATDNFPTPSGYTQIILTDARASSDNDKKARSTVLFIDGSGRVEICAKSDSRDICQYSEK

Nucleotide

• Length: 666 bp

>NTDB_id=400330 GJV52_RS10235 WP_095503772.1 2052603..2053268(-) (pilH) [Neisseria brasiliensis strain N.177.16]
ATGCACGCCAAACAAAAAGGTTTTACCCTTATCGAACTGTTGGTCGTGATTGCCATCATTGCAATCATGGCCGTCATCGC
CCTTCCCAACATGAGCCAATGGATCGCATCGCGCCGCGTGGCCAGCAATGCGGAACAAATCGCCAATCTTCTGCGCTTCG
CCCGCAGCGAAGCCGTTCGTCTAAATATTCCAGTTTATGTTTGTCCGGTTCAAATTAAAAAAGATGGCAAATCTAATGGT
AGTTGTAATGCCAGTTTCGGTAACCAAGGTTTGATGGCTTTTGCCGATACCGATAAAAACAAACAATATCAACGCACTTC
TACCGATATTCAATTACGCGCTATCATCTTAAACAGTAAAGATAATGATCGCATAACCCATAGCTATTATGCTTACCCCT
TTAGCGGCAGCAAAACCACCGATAGCAACGTCCGCTGGACTTTCCTACCTAACGGTAATTTTGTACGTTGGAATAAATCT
CCTACTGCGACAGACAATTTCCCGACACCTAGCGGTTACACTCAAATTATCTTAACTGATGCCCGTGCAAGCAGCGATAA
TGATAAAAAAGCCCGCTCTACCGTATTGTTTATCGACGGCAGCGGCCGTGTAGAAATCTGCGCCAAATCCGACTCGCGTG
ATATTTGCCAATATTCCGAAAAATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (4-28)

• Type II transport protein GspH (GspH) (44-166)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilH	Neisseria gonorrhoeae MS11	54.378	98.19	0.534