Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HUF04_RS28480 Genome accession   NZ_CP054581
Coordinates   5957057..5957482 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain YTSEY8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5952057..5962482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUF04_RS28465 (HUF04_28510) pilX 5952627..5953214 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  HUF04_RS28470 (HUF04_28515) pilY1 5953226..5956711 (+) 3486 WP_034040518.1 type 4a pilus biogenesis protein PilY1 -
  HUF04_RS28475 (HUF04_28520) pilY2 5956713..5957060 (+) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  HUF04_RS28480 (HUF04_28525) comF 5957057..5957482 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HUF04_RS28485 (HUF04_28530) ispH 5957529..5958473 (-) 945 WP_034040515.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HUF04_RS28490 (HUF04_28535) fkpB 5958559..5958999 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HUF04_RS28495 (HUF04_28540) lspA 5958992..5959501 (-) 510 WP_003102615.1 signal peptidase II -
  HUF04_RS28500 (HUF04_28545) ileS 5959494..5962325 (-) 2832 WP_023103515.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=400302 HUF04_RS28480 WP_003094721.1 5957057..5957482(+) (comF) [Pseudomonas aeruginosa strain YTSEY8]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=400302 HUF04_RS28480 WP_003094721.1 5957057..5957482(+) (comF) [Pseudomonas aeruginosa strain YTSEY8]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383