Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HPE49_RS12440 Genome accession   NZ_CP054363
Coordinates   2569436..2570200 (+) Length   254 a.a.
NCBI ID   WP_001136206.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-171     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2564436..2575200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE49_RS12420 (HPE49_12415) acpT 2565390..2565977 (+) 588 WP_000285764.1 4'-phosphopantetheinyl transferase AcpT -
  HPE49_RS12425 (HPE49_12420) nikA 2566088..2567662 (+) 1575 WP_000953341.1 nickel ABC transporter substrate-binding protein -
  HPE49_RS12430 (HPE49_12425) nikB 2567662..2568606 (+) 945 WP_000947063.1 nickel ABC transporter permease subunit NikB -
  HPE49_RS12435 (HPE49_12430) nikC 2568603..2569436 (+) 834 WP_042105887.1 nickel ABC transporter permease subunit NikC -
  HPE49_RS12440 (HPE49_12435) amiE 2569436..2570200 (+) 765 WP_001136206.1 nickel import ATP-binding protein NikD Regulator
  HPE49_RS12445 (HPE49_12440) nikE 2570197..2571003 (+) 807 WP_000173650.1 nickel import ATP-binding protein NikE -
  HPE49_RS12450 (HPE49_12445) nikR 2571009..2571410 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HPE49_RS12455 (HPE49_12450) - 2571530..2571889 (-) 360 WP_000593557.1 type II toxin-antitoxin system HicB family antitoxin -
  HPE49_RS12460 (HPE49_12455) - 2571886..2572161 (-) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  HPE49_RS12465 (HPE49_12460) yhhJ 2572234..2573358 (-) 1125 WP_001318088.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26810.47 Da        Isoelectric Point: 6.9016

>NTDB_id=399047 HPE49_RS12440 WP_001136206.1 2569436..2570200(+) (amiE) [Escherichia coli strain SCU-171]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHVVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=399047 HPE49_RS12440 WP_001136206.1 2569436..2570200(+) (amiE) [Escherichia coli strain SCU-171]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGCATTCTCC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACTACCGACCTTGATGTGGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAGAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGATGTGGCGGTGA
TGTCGCACGGTAAAATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGTGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment