Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HR073_RS20140 Genome accession   NZ_CP054227
Coordinates   4254864..4255628 (+) Length   254 a.a.
NCBI ID   WP_001136197.1    Uniprot ID   -
Organism   Escherichia coli strain EcPF15     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4249864..4260628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR073_RS20120 (HR073_20110) acpT 4250818..4251405 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  HR073_RS20125 (HR073_20115) nikA 4251516..4253090 (+) 1575 WP_000493125.1 nickel ABC transporter substrate-binding protein -
  HR073_RS20130 (HR073_20120) nikB 4253090..4254034 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  HR073_RS20135 (HR073_20125) nikC 4254031..4254864 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  HR073_RS20140 (HR073_20130) amiE 4254864..4255628 (+) 765 WP_001136197.1 nickel import ATP-binding protein NikD Regulator
  HR073_RS20145 (HR073_20135) nikE 4255625..4256431 (+) 807 WP_000173652.1 nickel import ATP-binding protein NikE -
  HR073_RS20150 (HR073_20140) nikR 4256437..4256838 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HR073_RS20155 (HR073_20145) - 4257037..4257783 (+) 747 WP_001329803.1 GntR family transcriptional regulator -
  HR073_RS20160 (HR073_20150) - 4257808..4258281 (+) 474 WP_001306344.1 PTS sugar transporter subunit IIA -
  HR073_RS20165 (HR073_20155) - 4258278..4258559 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  HR073_RS20170 (HR073_20160) - 4258636..4259994 (+) 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26813.43 Da        Isoelectric Point: 6.6886

>NTDB_id=398359 HR073_RS20140 WP_001136197.1 4254864..4255628(+) (amiE) [Escherichia coli strain EcPF15]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=398359 HR073_RS20140 WP_001136197.1 4254864..4255628(+) (amiE) [Escherichia coli strain EcPF15]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402