Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQN50_RS28480 Genome accession   NZ_CP053922
Coordinates   6096112..6097896 (+) Length   594 a.a.
NCBI ID   WP_003096276.1    Uniprot ID   A0A0H2ZIS8
Organism   Pseudomonas aeruginosa strain YD001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6091112..6102896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN50_RS28460 argE 6091352..6092506 (-) 1155 WP_003100077.1 acetylornithine deacetylase -
  HQN50_RS28465 - 6092606..6093874 (-) 1269 WP_003100079.1 inorganic phosphate transporter -
  HQN50_RS28470 - 6093919..6094596 (-) 678 WP_003100081.1 TIGR00153 family protein -
  HQN50_RS28475 - 6094710..6096074 (+) 1365 WP_023088673.1 inorganic triphosphatase -
  HQN50_RS28480 pilB 6096112..6097896 (+) 1785 WP_003096276.1 GspE/PulE family protein Machinery gene
  HQN50_RS28485 - 6098118..6098570 (+) 453 WP_009314473.1 NfeD family protein -
  HQN50_RS28490 - 6098702..6099031 (+) 330 WP_003096280.1 DUF2388 domain-containing protein -
  HQN50_RS28495 gcvP 6099066..6101942 (-) 2877 WP_017148668.1 aminomethyl-transferring glycine dehydrogenase -
  HQN50_RS28500 gcvH 6102116..6102505 (-) 390 WP_031276264.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 65981.61 Da        Isoelectric Point: 6.5096

>NTDB_id=396834 HQN50_RS28480 WP_003096276.1 6096112..6097896(+) (pilB) [Pseudomonas aeruginosa strain YD001]
MSVFATAAQDRLLDLGDLLRELVAQGRVGQEQAEQCLAIRRSAVRNQQHPLEFLAAQQVEDLKRPGRKLDLETLTQWLAE
YAGQPYLRIDPLKIDVAAVTPLMSYAFAQRHGILAVALSPEEVTIASAQPFVQGWESNLVHVLKRPIKRVVANPADIQRY
TVEFFRLARSVSGATSPDQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAVVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGAEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
FDQLGFSADDLRRWQSMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATDEVNVCTIEDPIEMIEPSFNQMQVQHNIDLTF
ASGVRALLRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSPTAITRLLELGVPYYLIRATVLGVMAQRLVRT
LCPHCKAPMQLADSDWHELTRPWSAPPPSGAHQAVGCLECRDTGYRGRAGVYEIMLLSDSLKELITADTDLVALRRQAYK
EGTRSLRLSGAQKVAAGISTLEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=396834 HQN50_RS28480 WP_003096276.1 6096112..6097896(+) (pilB) [Pseudomonas aeruginosa strain YD001]
ATGTCCGTGTTCGCCACCGCCGCCCAGGACCGCCTGCTGGACCTAGGCGACCTGCTCCGCGAGCTGGTCGCCCAGGGCCG
GGTCGGGCAGGAGCAGGCCGAACAGTGCCTGGCGATCCGCCGCAGCGCGGTGCGCAACCAGCAGCATCCGCTGGAGTTCC
TCGCCGCGCAGCAGGTCGAGGATCTCAAGCGCCCCGGACGCAAGCTCGATCTGGAGACCCTCACCCAGTGGCTGGCCGAG
TACGCCGGCCAGCCCTACCTGCGCATCGACCCGCTGAAGATCGACGTCGCCGCGGTGACTCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCATGGCATCCTCGCCGTCGCGCTGAGTCCGGAGGAAGTCACCATCGCCAGCGCCCAGCCCTTCGTACAGG
GCTGGGAAAGCAACCTGGTGCATGTGCTCAAGCGACCAATCAAGCGGGTGGTGGCGAACCCGGCGGACATCCAGCGCTAC
ACCGTCGAGTTCTTCCGCCTGGCCCGCTCGGTCAGCGGCGCCACCTCGCCGGACCAGAAGATCAGCGGCGTCGGCAACTT
CGAGCAGTTGCTCAACCTCGGCGCCGCCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGCAGGGCACCGTGCGCTTCCGT
ATCGACGGCGTGCTGCACAACGTCTACCAGTTCCCGCCGCAGGTGACCATGGCGGTGGTCAGCCGCCTGAAGTCGCTGGG
ACGGATGAACGTGGCGGAGAAACGCAAGCCGCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGCCGAGGTGGAGC
TGCGCCTGTCGACCCTGCCCACCGCCTTCGGCGAGAAGATGGTGATGCGCATCTTCGACCCCGAGGTGCTGCTCAAGAGC
TTCGACCAGCTCGGCTTCTCCGCCGACGACCTGCGCCGCTGGCAGAGCATGACCAGCCAGCCCAACGGCATCATCCTGGT
CACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTCTACACCACGCTCAAGCAACTGGCCACCGATGAGGTGAACGTCT
GCACCATCGAGGACCCGATCGAGATGATCGAGCCCTCGTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCAGCGGCGTACGCGCCCTGCTGCGGCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGA
GATGGCGATCCAGGCGGCGCTCACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACTCGCCCACGGCGATCACCC
GCCTGCTCGAACTGGGCGTGCCCTACTACCTGATCCGCGCCACGGTGCTCGGGGTCATGGCCCAGCGCCTGGTGCGCACA
CTCTGCCCGCACTGCAAGGCGCCGATGCAACTGGCGGACAGCGACTGGCACGAACTGACCCGCCCCTGGAGCGCCCCGCC
GCCCAGCGGCGCGCACCAGGCCGTGGGTTGCCTGGAGTGCCGCGACACCGGCTATCGCGGGCGCGCCGGCGTCTATGAAA
TCATGCTGCTCAGCGATAGCCTGAAAGAACTGATCACCGCCGACACCGACCTGGTCGCCCTGCGCCGCCAGGCGTACAAG
GAAGGCACCCGCAGCCTGCGCCTGTCCGGCGCGCAGAAGGTCGCCGCGGGTATCAGTACGCTGGAGGAAGTACTGCGGGT
GACCCCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.682

90.067

0.375

Multiple sequence alignment