Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   GFH33_RS23550 Genome accession   NZ_CP045585
Coordinates   4550673..4553012 (-) Length   779 a.a.
NCBI ID   WP_000941236.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain x023     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4545673..4558012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH33_RS23530 (GFH33_23530) - 4546920..4547096 (-) 177 WP_001242605.1 hypothetical protein -
  GFH33_RS23535 (GFH33_23535) - 4547262..4547702 (-) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  GFH33_RS23540 (GFH33_23540) relA 4547719..4549902 (-) 2184 WP_001262811.1 GTP diphosphokinase -
  GFH33_RS23545 (GFH33_23545) - 4550113..4550625 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  GFH33_RS23550 (GFH33_23550) recJ 4550673..4553012 (-) 2340 WP_000941236.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  GFH33_RS23555 (GFH33_23555) - 4553114..4554007 (-) 894 WP_000409746.1 cation diffusion facilitator family transporter -
  GFH33_RS23560 (GFH33_23560) secDF 4554164..4556428 (-) 2265 WP_001119072.1 protein translocase subunit SecDF -
  GFH33_RS23565 (GFH33_23565) - 4556698..4556982 (-) 285 WP_000886978.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88159.72 Da        Isoelectric Point: 4.9368

>NTDB_id=395344 GFH33_RS23550 WP_000941236.1 4550673..4553012(-) (recJ) [Bacillus thuringiensis strain x023]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENKFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGI
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLNQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=395344 GFH33_RS23550 WP_000941236.1 4550673..4553012(-) (recJ) [Bacillus thuringiensis strain x023]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCATTTCGTTGGGCGC
ATAGTGCAGGTTTCTCACTGATTATTACTGTCGACACTGGCATCGCAGCAGTACATGAAGCGAATGTAGCGAAAGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCGCTTGCGATTATACATCCGAAATT
AGAAGGTGGCGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAGAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCTCGTATTAATGCGGTTGGGCGTTTAGAAGATGCTGCGC
CGGCTGTACACCTTTTATTATCAGATGATCCAGAGGAAGCGAAAGAGTTGGCCGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTTGAAAATAAATTCCCGCCAGAAGAAAACAAAGT
ATTAGTACTTGCAAAAGAAGGCTGGAACCCAGGGGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATCGATCCTATAAAAGAAACAGCAAAAGGCTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAACTTATCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGATTAAATGAACAAGCAGATGCGATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGCAAAGTAGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAAAAACTAGCCCCGTTTGGTATT
GGAAATCCGAAACCACGTATTGCAGTAAAAGATGCAGAGTTAGAAAGCATTCGCGCAATCGGATCAGATGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCTAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCATCCATCAATGAATGGAATAATTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACCGATGAATTACGTGAG
TTATTTAAGGCAGGATTCCCAGCGCGAATTTATACGCTATTTTATCAAGAGAATAACCACTTATTTAGTACAGTTCCTAC
GAGAGAACACTTTAAATGGTATTATTCTTTCTTAAACCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTTATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.455

99.358

0.521

Multiple sequence alignment