Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GCS91_RS30650 Genome accession   NZ_CP045291
Coordinates   6651576..6653192 (-) Length   538 a.a.
NCBI ID   WP_016454603.1    Uniprot ID   A0AAX3SLT0
Organism   Delftia tsuruhatensis strain TR1180     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6646576..6658192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCS91_RS30635 (GCS91_30635) - 6647538..6648035 (-) 498 WP_154836283.1 hypothetical protein -
  GCS91_RS30640 (GCS91_30640) - 6648092..6649231 (-) 1140 WP_154836284.1 slipin family protein -
  GCS91_RS30645 (GCS91_30645) rtcR 6649929..6651554 (+) 1626 WP_154836285.1 RNA repair transcriptional activator RtcR -
  GCS91_RS30650 (GCS91_30650) rpoN/rpoN1 6651576..6653192 (-) 1617 WP_016454603.1 RNA polymerase factor sigma-54 Machinery gene
  GCS91_RS30655 (GCS91_30655) lptB 6653247..6654044 (-) 798 WP_016450697.1 LPS export ABC transporter ATP-binding protein -
  GCS91_RS30660 (GCS91_30660) lptA 6654041..6654703 (-) 663 WP_016454602.1 lipopolysaccharide transport periplasmic protein LptA -
  GCS91_RS30665 (GCS91_30665) - 6654876..6655526 (-) 651 WP_016450695.1 thiol:disulfide interchange protein DsbA/DsbL -
  GCS91_RS30670 (GCS91_30670) - 6655608..6656288 (-) 681 WP_070080759.1 SPOR domain-containing protein -
  GCS91_RS30675 (GCS91_30675) argS 6656309..6658006 (-) 1698 WP_016450693.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 59267.33 Da        Isoelectric Point: 4.5784

>NTDB_id=393136 GCS91_RS30650 WP_016454603.1 6651576..6653192(-) (rpoN/rpoN1) [Delftia tsuruhatensis strain TR1180]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLGDNPFLELANDDGDHEAASSAPEPAAPGADAADSFQESR
ADQPSAAVEWEAPSDTSSGDAVETSGTAESLSWEGDGQTNSGNDDRDWGSDAPSSRTRGQGDDDETDATELARSAESLTD
HLHRQALSLRLGEVDRAALRFLIENLNDDGYLEDPLDELAQGLAGDDLEECEELVHRFTVALRLLQSLEPVGVGARDLAE
CLTLQLRDQLADAEREGDGSAEDEDRQAVLRTALDICAQPMDLLARRDLRKLASLCGAREERLREAMVLIARLEPRPGRR
FADVQRQIVVPDVIVRPVRMGAGRMEFSVQLNPDVMPKLRVHDIYASALRSHRGSEGHAALQQRLQEARWFIKNIQQRFD
TILRVSQAIVERQKNFFVHGELAMRPLVLRDIADALGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTEAGGNASST
AVRALIKQFVAAEDPRKPLSDNQLADMLKEQGIECARRTVAKYRELLKIPPTTLRKAL

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=393136 GCS91_RS30650 WP_016454603.1 6651576..6653192(-) (rpoN/rpoN1) [Delftia tsuruhatensis strain TR1180]
TTGAAGCCCGGACTGTCGCTGCGCGTCTCCCAGCATCTTTCGCTCACCCCGCAACTGCAGCAGTCGATACAGCTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAAGTGGAGCAGATGCTGGGCGACAACCCCTTCCTGGAGCTGGCGAACGACGACG
GCGACCACGAAGCGGCAAGCAGCGCCCCGGAGCCTGCGGCGCCCGGCGCCGACGCCGCGGACTCCTTCCAGGAAAGCCGT
GCGGACCAGCCATCGGCTGCGGTGGAATGGGAGGCGCCGTCGGACACATCCTCCGGCGATGCCGTCGAGACCTCCGGCAC
GGCCGAGTCCCTGAGCTGGGAAGGCGATGGCCAGACCAACTCGGGCAACGACGACCGCGACTGGGGCAGCGATGCGCCTT
CCAGCCGCACGCGCGGCCAGGGTGACGATGACGAGACCGATGCCACCGAGCTGGCACGCAGCGCAGAAAGCCTGACCGAC
CACCTGCACCGCCAGGCCCTGAGCCTGCGCCTGGGCGAGGTGGACCGCGCCGCGCTGCGCTTTCTGATCGAGAACCTCAA
CGACGACGGCTACCTGGAAGACCCGCTCGACGAGCTGGCACAGGGCCTGGCGGGCGACGATCTCGAGGAGTGCGAGGAAC
TGGTGCACCGCTTCACCGTGGCGCTGCGCCTGTTGCAAAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGACCTGGCCGAA
TGCCTGACGCTGCAGCTGCGCGACCAGCTGGCCGATGCCGAGCGCGAAGGCGACGGCAGTGCCGAGGACGAAGATCGGCA
GGCCGTGCTGCGCACCGCGCTGGACATCTGCGCCCAGCCCATGGACCTGCTGGCGCGGCGTGACCTGCGCAAGCTCGCCA
GCCTGTGCGGCGCGCGCGAGGAGCGGCTGCGCGAGGCGATGGTCCTGATCGCCCGGCTGGAGCCGCGCCCGGGCCGGCGC
TTCGCCGACGTGCAGCGCCAGATCGTCGTGCCCGATGTCATCGTGCGCCCGGTGCGCATGGGTGCGGGACGCATGGAGTT
CAGCGTGCAACTCAACCCCGATGTCATGCCCAAGCTGCGCGTGCACGACATCTATGCCAGCGCGCTGCGCAGCCACCGGG
GCAGCGAAGGGCATGCGGCGTTGCAGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGAC
ACCATCCTGCGCGTGTCGCAGGCCATCGTGGAGCGCCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGCCCGCT
GGTGCTGCGCGACATTGCCGATGCGCTGGGCCTGCATGAATCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCA
CGCCGCAGGGCACCTACGAACTCAAGTATTTCTTCGGCTCGGGGCTGGGCACCGAGGCGGGCGGCAACGCGTCGAGCACG
GCCGTGCGCGCGCTCATCAAGCAGTTCGTCGCAGCCGAGGACCCCAGGAAGCCGCTGTCGGACAACCAGCTGGCCGACAT
GCTCAAGGAGCAGGGCATCGAATGCGCACGGCGCACCGTGGCCAAGTACCGCGAACTGCTCAAGATCCCCCCGACCACGC
TGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-365)

Sigma-54, DNA binding domain (Sigma54_DBD)

(380-536)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.111

100

0.513

Multiple sequence alignment