Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   FDZ14_RS03985 Genome accession   NZ_CP045272
Coordinates   647365..649707 (+) Length   780 a.a.
NCBI ID   WP_171776594.1    Uniprot ID   A0A6M6DVR9
Organism   Priestia megaterium strain FDU301     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 642365..654707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDZ14_RS03970 (FDZ14_03975) spoVB 642881..644431 (-) 1551 WP_171776592.1 stage V sporulation protein B -
  FDZ14_RS03975 (FDZ14_03980) - 644556..644849 (+) 294 WP_016765633.1 post-transcriptional regulator -
  FDZ14_RS03980 (FDZ14_03985) secDF 644987..647224 (+) 2238 WP_171776593.1 protein translocase subunit SecDF -
  FDZ14_RS03985 (FDZ14_03990) recJ 647365..649707 (+) 2343 WP_171776594.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  FDZ14_RS03990 (FDZ14_03995) - 649723..650235 (+) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  FDZ14_RS03995 (FDZ14_04000) - 650421..652619 (+) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  FDZ14_RS04000 (FDZ14_04005) dtd 652635..653096 (+) 462 WP_016765630.1 D-aminoacyl-tRNA deacylase -
  FDZ14_RS04005 (FDZ14_04010) - 653087..653971 (-) 885 WP_253948205.1 N-acetylmuramoyl-L-alanine amidase -
  FDZ14_RS04010 (FDZ14_04015) - 654138..654314 (+) 177 WP_013059305.1 hypothetical protein -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 88007.62 Da        Isoelectric Point: 4.6748

>NTDB_id=393047 FDZ14_RS03985 WP_171776594.1 647365..649707(+) (recJ) [Priestia megaterium strain FDU301]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETEYAREL
DFDLIITDHHEPGPILPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKV
RQQIVNDITEEAVEFVENMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSDVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKECMFAGEQIVLLDLPPLQ
KWLECLFVQGIPSRLYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIQ

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=393047 FDZ14_RS03985 WP_171776594.1 647365..649707(+) (recJ) [Priestia megaterium strain FDU301]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACATTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCGGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCTGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGTTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGAGTATGCACGTGAATTA
GATTTTGATTTAATTATTACGGATCACCACGAGCCGGGGCCGATTTTACCGGATGCGTATGCAATTATTCATCCAAAGCT
ACCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGTA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGTGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTACATACAACATCTATGCGGAAAATTGGCGCTAATAAAAATCATTT
AAAACTGGTGTTTGAAGACAGCGATGTATCGGTTGACGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCGTTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGAATGTATGTTTGCAGGGGAACAAATCGTTTTATTAGATTTGCCTCCTTTACAG
AAGTGGCTTGAATGTTTGTTTGTCCAAGGTATTCCTTCTAGATTATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTCAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M6DVR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.084

99.615

0.549

Multiple sequence alignment