Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GXM_RS06025 Genome accession   NZ_CP045226
Coordinates   1348754..1349860 (+) Length   368 a.a.
NCBI ID   WP_152590705.1    Uniprot ID   -
Organism   Nostoc sphaeroides CCNUC1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1343754..1354860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GXM_RS06015 (GXM_01488) - 1344545..1347058 (+) 2514 WP_152588464.1 beta strand repeat-containing protein -
  GXM_RS06020 (GXM_01489) - 1347146..1348450 (-) 1305 WP_152588465.1 ABC transporter substrate-binding protein -
  GXM_RS06025 (GXM_01490) pilM 1348754..1349860 (+) 1107 WP_152590705.1 type IV pilus assembly protein PilM Machinery gene
  GXM_RS06030 (GXM_01491) - 1349865..1350641 (+) 777 WP_152588466.1 PilN domain-containing protein -
  GXM_RS06035 (GXM_01492) - 1350638..1351411 (+) 774 WP_152588467.1 pilus assembly protein PilO -
  GXM_RS06040 (GXM_01493) - 1351684..1354212 (+) 2529 WP_152588468.1 type IV pilus secretin family protein -
  GXM_RS46645 (GXM_01494) - 1354465..1354617 (+) 153 WP_338115370.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40468.61 Da        Isoelectric Point: 4.4661

>NTDB_id=392834 GXM_RS06025 WP_152590705.1 1348754..1349860(+) (pilM) [Nostoc sphaeroides CCNUC1]
MVKSFKSLFSKSKKGVGIELAPERVNVVQLRKQGQGLKLETFTSVAVPEGVVTDGQITDPAAMAQLIQQALTESKIKTSR
VATGVPGRDSIVRIIPVPAELDDKELREMVLNHEAGLYLPYPREEADVDYQKLGYKMDEDGIEKVEVLLVATRKEITDTY
ISTFEQAGLQIDILEINSFALIRTIREQLRQFGPQEAAVLVDIEFDSTEIAIIVNGVPQFSRTVPIGTYQMQMALARAMS
LPTSRDMELLHGMVIPPTSLDGGKTGVTEINPGMAAILRVLGELTDELRRSIDFYLNQTENMEVAQIILAGPGGGLQQLD
EFFTQRLSLPTTQIDPIGALSLEIDAEKYPQVERSGLAIVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=392834 GXM_RS06025 WP_152590705.1 1348754..1349860(+) (pilM) [Nostoc sphaeroides CCNUC1]
GTGGTAAAAAGCTTCAAAAGTCTGTTTAGCAAATCAAAAAAAGGGGTCGGCATTGAACTTGCTCCCGAACGGGTGAATGT
AGTTCAGCTACGCAAGCAGGGTCAAGGCTTGAAGCTAGAAACCTTTACATCGGTAGCAGTTCCAGAAGGCGTAGTTACCG
ATGGTCAAATCACCGACCCCGCAGCAATGGCGCAATTAATCCAACAGGCGCTAACTGAGAGCAAAATCAAAACTTCTCGC
GTTGCCACTGGTGTACCAGGGCGAGATTCCATCGTTCGGATTATACCAGTGCCAGCAGAGTTAGATGATAAAGAACTGCG
AGAAATGGTTCTCAACCATGAAGCAGGTTTATATTTACCCTATCCTCGTGAAGAGGCTGATGTAGATTATCAGAAACTTG
GGTACAAGATGGATGAGGATGGCATAGAAAAGGTAGAGGTGCTTTTGGTTGCCACTCGTAAGGAAATTACTGATACCTAT
ATAAGTACCTTCGAGCAGGCAGGATTACAAATCGATATTTTAGAAATTAACAGTTTTGCTCTGATTCGGACTATTCGTGA
GCAATTGCGACAATTTGGGCCGCAAGAAGCAGCAGTGCTAGTTGATATAGAGTTCGACAGTACAGAAATCGCCATCATCG
TTAACGGAGTGCCGCAATTTTCGCGCACAGTCCCAATCGGGACTTATCAAATGCAAATGGCCTTAGCAAGGGCAATGAGC
TTACCCACATCACGAGATATGGAACTGTTACACGGAATGGTCATTCCCCCAACTTCTCTAGACGGCGGGAAAACTGGGGT
TACTGAAATCAATCCTGGTATGGCAGCCATATTGAGAGTATTGGGAGAACTAACGGATGAACTGCGCCGTTCCATCGATT
TTTACCTAAATCAAACTGAAAATATGGAGGTAGCGCAGATTATATTAGCTGGGCCAGGAGGTGGACTCCAACAGCTAGAT
GAATTCTTTACCCAACGATTGAGTTTGCCAACTACCCAAATAGATCCAATCGGCGCTTTGTCGTTGGAAATTGACGCTGA
GAAATATCCACAGGTAGAACGCTCTGGCTTGGCAATCGTACTTGGTCTAGGAATGCGGGAGGTGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-238)

Type IV pilus assembly protein PilM; (PilM_2)

(283-367)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

66.213

99.728

0.66

Multiple sequence alignment