Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   F9277_RS00750 Genome accession   NZ_CP045070
Coordinates   151844..152860 (+) Length   338 a.a.
NCBI ID   WP_152163015.1    Uniprot ID   -
Organism   Vibrio harveyi strain WXL538     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146844..157860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9277_RS00735 (F9277_00735) - 147217..147945 (-) 729 WP_005451494.1 redoxin family protein -
  F9277_RS00740 (F9277_00740) oxyR 148120..149028 (+) 909 WP_152163013.1 DNA-binding transcriptional regulator OxyR -
  F9277_RS00745 (F9277_00745) - 149095..151665 (-) 2571 WP_152163014.1 penicillin-binding protein 1A -
  F9277_RS00750 (F9277_00750) pilM 151844..152860 (+) 1017 WP_152163015.1 type IV pilus assembly protein PilM Machinery gene
  F9277_RS00755 (F9277_00755) pilN 152844..153419 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  F9277_RS00760 (F9277_00760) pilO 153412..154005 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  F9277_RS00765 (F9277_00765) pilP 153995..154510 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  F9277_RS00770 (F9277_00770) pilQ 154545..156296 (+) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  F9277_RS00775 (F9277_00775) aroK 156486..157004 (+) 519 WP_005451507.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38067.46 Da        Isoelectric Point: 6.4209

>NTDB_id=391651 F9277_RS00750 WP_152163015.1 151844..152860(+) (pilM) [Vibrio harveyi strain WXL538]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVERVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=391651 F9277_RS00750 WP_152163015.1 151844..152860(+) (pilM) [Vibrio harveyi strain WXL538]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAACGCT
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGCGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGAACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGAGCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGTAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

91.124

100

0.911
  pilM Vibrio cholerae strain A1552

48.538

100

0.491

Multiple sequence alignment