Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HLK60_RS01185 Genome accession   NZ_CP053296
Coordinates   272305..273069 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain M1/5     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 267305..278069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLK60_RS01170 (HLK60_01170) yhhJ 269962..271086 (+) 1125 WP_001314210.1 ABC transporter permease -
  HLK60_RS01175 (HLK60_01175) nikR 271095..271496 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HLK60_RS01180 (HLK60_01180) nikE 271502..272308 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  HLK60_RS01185 (HLK60_01185) amiE 272305..273069 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  HLK60_RS01190 (HLK60_01190) nikC 273069..273902 (-) 834 WP_001008955.1 nickel ABC transporter permease subunit NikC -
  HLK60_RS01195 (HLK60_01195) nikB 273899..274843 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  HLK60_RS01200 (HLK60_01200) nikA 274843..276417 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  HLK60_RS01205 (HLK60_01205) acpT 276528..277115 (-) 588 WP_000285790.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=391504 HLK60_RS01185 WP_001136232.1 272305..273069(-) (amiE) [Escherichia coli strain M1/5]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=391504 HLK60_RS01185 WP_001136232.1 272305..273069(-) (amiE) [Escherichia coli strain M1/5]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment