Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HHJ54_RS07245 Genome accession   NZ_CP053245
Coordinates   1534800..1535564 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain SCU-485     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1529800..1540564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ54_RS07225 (HHJ54_07225) acpT 1530754..1531341 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  HHJ54_RS07230 (HHJ54_07230) nikA 1531452..1533026 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  HHJ54_RS07235 (HHJ54_07235) nikB 1533026..1533970 (+) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  HHJ54_RS07240 (HHJ54_07240) nikC 1533967..1534800 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  HHJ54_RS07245 (HHJ54_07245) amiE 1534800..1535564 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  HHJ54_RS07250 (HHJ54_07250) nikE 1535561..1536367 (+) 807 WP_000173677.1 nickel import ATP-binding protein NikE -
  HHJ54_RS07255 (HHJ54_07255) nikR 1536373..1536774 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HHJ54_RS07260 (HHJ54_07260) - 1536973..1537719 (+) 747 WP_001296489.1 UTRA domain-containing protein -
  HHJ54_RS07265 (HHJ54_07265) - 1537744..1538217 (+) 474 WP_001161647.1 PTS sugar transporter subunit IIA -
  HHJ54_RS07270 (HHJ54_07270) - 1538214..1538495 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  HHJ54_RS07275 (HHJ54_07275) - 1538572..1539930 (+) 1359 WP_001304938.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=390912 HHJ54_RS07245 WP_001136232.1 1534800..1535564(+) (amiE) [Escherichia coli strain SCU-485]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=390912 HHJ54_RS07245 WP_001136232.1 1534800..1535564(+) (amiE) [Escherichia coli strain SCU-485]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCA
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398