Detailed information NTG0389974

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: F7V86_RS07595
• Genome accession: NZ_CP044444
• Coordinates: 1449724..1451103 (+)
• Length: 459 a.a.
• NCBI ID: WP_014416742.1
• Uniprot ID: I2C0L1
• Organism: Bacillus amyloliquefaciens strain KC41
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1444724..1456103

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F7V86_RS07575	ctsR	1445072..1445536 (+)	465	WP_003156396.1	transcriptional regulator CtsR	-
F7V86_RS07580	-	1445550..1446107 (+)	558	WP_007410387.1	UvrB/UvrC motif-containing protein	-
F7V86_RS07585	-	1446107..1447198 (+)	1092	WP_003156398.1	protein arginine kinase	-
F7V86_RS07590	clpC	1447195..1449630 (+)	2436	WP_017419467.1	ATP-dependent protease ATP-binding subunit ClpC	Regulator
F7V86_RS07595	radA	1449724..1451103 (+)	1380	WP_014416742.1	DNA repair protein RadA	Machinery gene
F7V86_RS07600	disA	1451107..1452189 (+)	1083	WP_003156401.1	DNA integrity scanning diadenylate cyclase DisA	-
F7V86_RS07605	-	1452303..1453403 (+)	1101	WP_003156403.1	PIN/TRAM domain-containing protein	-
F7V86_RS07610	ispD	1453416..1454114 (+)	699	WP_025650291.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
F7V86_RS07615	ispF	1454107..1454583 (+)	477	WP_003156407.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-

Sequence

Protein

• Length: 459 a.a.
• Molecular weight: 49547.98 Da
• Isoelectric Point: 7.1760

>NTDB_id=389974 F7V86_RS07595 WP_014416742.1 1449724..1451103(+) (radA) [Bacillus amyloliquefaciens strain KC41]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide

• Length: 1380 bp

>NTDB_id=389974 F7V86_RS07595 WP_014416742.1 1449724..1451103(+) (radA) [Bacillus amyloliquefaciens strain KC41]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCGGAAGAACCCCGCGTTCACACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGTGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTGTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAGGTGCTGAACCCTTCTGAAATATTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTGTCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCTGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (364-430)

• AAA domain (AAA_25) (73-222)

3D structure

• AlphaFold DB: I2C0L1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	93.45	99.782	0.932
radA	Streptococcus pneumoniae Rx1	63.797	98.693	0.63
radA	Streptococcus pneumoniae D39	63.797	98.693	0.63
radA	Streptococcus pneumoniae R6	63.797	98.693	0.63
radA	Streptococcus pneumoniae TIGR4	63.797	98.693	0.63
radA	Streptococcus mitis NCTC 12261	63.797	98.693	0.63
radA	Streptococcus mitis SK321	63.576	98.693	0.627