Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HKW69_RS01965 Genome accession   NZ_CP053080
Coordinates   406423..407187 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain HB37     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 401423..412187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW69_RS01950 (HKW69_01955) yhhJ 404080..405204 (+) 1125 WP_001297994.1 ABC transporter permease -
  HKW69_RS01955 (HKW69_01960) nikR 405213..405614 (-) 402 WP_001190060.1 nickel-responsive transcriptional regulator NikR -
  HKW69_RS01960 (HKW69_01965) nikE 405620..406426 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  HKW69_RS01965 (HKW69_01970) amiE 406423..407187 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  HKW69_RS01970 (HKW69_01975) nikC 407187..408020 (-) 834 WP_001008959.1 nickel ABC transporter permease subunit NikC -
  HKW69_RS01975 (HKW69_01980) nikB 408017..408961 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  HKW69_RS01980 (HKW69_01985) nikA 408961..410535 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  HKW69_RS01985 (HKW69_01990) acpT 410646..411233 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=389693 HKW69_RS01965 WP_001136232.1 406423..407187(-) (amiE) [Escherichia coli strain HB37]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=389693 HKW69_RS01965 WP_001136232.1 406423..407187(-) (amiE) [Escherichia coli strain HB37]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGAAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment