Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HHJ21_RS20535 Genome accession   NZ_CP051753
Coordinates   4332491..4333255 (+) Length   254 a.a.
NCBI ID   WP_001136200.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-102     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4327491..4338255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ21_RS20515 (HHJ21_20510) acpT 4328445..4329032 (+) 588 WP_000285767.1 4'-phosphopantetheinyl transferase AcpT -
  HHJ21_RS20520 (HHJ21_20515) nikA 4329143..4330717 (+) 1575 WP_000953346.1 nickel ABC transporter substrate-binding protein -
  HHJ21_RS20525 (HHJ21_20520) nikB 4330717..4331661 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  HHJ21_RS20530 (HHJ21_20525) nikC 4331658..4332491 (+) 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
  HHJ21_RS20535 (HHJ21_20530) amiE 4332491..4333255 (+) 765 WP_001136200.1 nickel import ATP-binding protein NikD Regulator
  HHJ21_RS20540 (HHJ21_20535) nikE 4333252..4334058 (+) 807 WP_000173688.1 nickel import ATP-binding protein NikE -
  HHJ21_RS20545 (HHJ21_20540) nikR 4334064..4334465 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HHJ21_RS20550 (HHJ21_20545) - 4334585..4334944 (-) 360 WP_000593557.1 type II toxin-antitoxin system HicB family antitoxin -
  HHJ21_RS20555 (HHJ21_20550) - 4334941..4335216 (-) 276 WP_001260302.1 type II toxin-antitoxin system HicA family toxin -
  HHJ21_RS20560 (HHJ21_20555) yhhJ 4335289..4336413 (-) 1125 WP_001366710.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26783.36 Da        Isoelectric Point: 6.4896

>NTDB_id=388035 HHJ21_RS20535 WP_001136200.1 4332491..4333255(+) (amiE) [Escherichia coli strain SCU-102]
MPQQIELRDIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=388035 HHJ21_RS20535 WP_001136200.1 4332491..4333255(+) (amiE) [Escherichia coli strain SCU-102]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGTGTATCGTTAACCCTGCA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGCATTCTCC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGTGGCGGTATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACTACCGACCTTGATGTGGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAGAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGATGTGGCGGTGA
TGTCGCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402