Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HHJ31_RS12310 Genome accession   NZ_CP051719
Coordinates   2543072..2543836 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain SCU-116     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2538072..2548836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ31_RS12290 (HHJ31_12290) acpT 2539026..2539613 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  HHJ31_RS12295 (HHJ31_12295) nikA 2539724..2541298 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  HHJ31_RS12300 (HHJ31_12300) nikB 2541298..2542242 (+) 945 WP_000947074.1 nickel ABC transporter permease subunit NikB -
  HHJ31_RS12305 (HHJ31_12305) nikC 2542239..2543072 (+) 834 WP_001514028.1 nickel ABC transporter permease subunit NikC -
  HHJ31_RS12310 (HHJ31_12310) amiE 2543072..2543836 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  HHJ31_RS12315 (HHJ31_12315) nikE 2543833..2544639 (+) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  HHJ31_RS12320 (HHJ31_12320) nikR 2544645..2545046 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HHJ31_RS12325 (HHJ31_12325) - 2545245..2545991 (+) 747 WP_001332161.1 GntR family transcriptional regulator -
  HHJ31_RS12330 (HHJ31_12330) - 2546016..2546489 (+) 474 WP_001161647.1 PTS sugar transporter subunit IIA -
  HHJ31_RS12335 (HHJ31_12335) - 2546486..2546767 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  HHJ31_RS12340 (HHJ31_12340) - 2546844..2548202 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=387328 HHJ31_RS12310 WP_001136232.1 2543072..2543836(+) (amiE) [Escherichia coli strain SCU-116]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=387328 HHJ31_RS12310 WP_001136232.1 2543072..2543836(+) (amiE) [Escherichia coli strain SCU-116]
ATGCCACAACAGATTGAGCTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCA
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398