Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EVS87_RS05380 Genome accession   NZ_CP043559
Coordinates   1040229..1043738 (+) Length   1169 a.a.
NCBI ID   WP_135009036.1    Uniprot ID   -
Organism   Bacillus altitudinis strain CHB19     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1035229..1048738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVS87_RS05350 (EVS87_005350) lepB 1035736..1036248 (+) 513 WP_007499139.1 signal peptidase I -
  EVS87_RS05355 (EVS87_005355) - 1036284..1037426 (-) 1143 WP_007499137.1 AbrB family transcriptional regulator -
  EVS87_RS05360 (EVS87_005360) - 1037703..1038125 (+) 423 WP_007499135.1 BlaI/MecI/CopY family transcriptional regulator -
  EVS87_RS05365 (EVS87_005365) - 1038118..1038957 (+) 840 WP_058337506.1 M56 family metallopeptidase -
  EVS87_RS05370 (EVS87_005370) - 1039015..1039425 (+) 411 WP_008348626.1 DoxX family protein -
  EVS87_RS05375 (EVS87_005375) - 1039616..1040062 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  EVS87_RS05380 (EVS87_005380) addB 1040229..1043738 (+) 3510 WP_135009036.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EVS87_RS05385 (EVS87_005385) addA 1043716..1047420 (+) 3705 WP_135009035.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EVS87_RS05390 (EVS87_005390) sbcD 1047446..1048621 (+) 1176 WP_041507491.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134646.63 Da        Isoelectric Point: 5.2365

>NTDB_id=385093 EVS87_RS05380 WP_135009036.1 1040229..1043738(+) (addB) [Bacillus altitudinis strain CHB19]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSQLYQLAKECGAAISENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVRGEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVVQLMDEFVEMLGEEEL
SFPLFQQMMDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFVIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHYLYLSYSASDTEGRSLLPSTLIKYCEELMPNHQQALYVLDPEQLDDQEQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNRDVSKKIMSSLFFTNRAKRLNPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVIYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLGEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=385093 EVS87_RS05380 WP_135009036.1 1040229..1043738(+) (addB) [Bacillus altitudinis strain CHB19]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATTCTAGAAGAAATCAAAGAACAACTGCG
GCTTGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGGGCCAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCGTCAATTTGTCACAAGCACAGGGATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGATTTGTTGAACAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCGGAGGATATTGAAAAAATCTCGGCATCCAGTATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTGCAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAGGGGCACACATTTATATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTTTTGCTACATGCCGAAAAAATAACAGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCAAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCCCAGCTTTATCAGTTGGCTAA
AGAGTGCGGAGCGGCTATTTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACCCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACCTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTAAGAGGGGAAGGTCTTAGGTTACGAGACATCTCAAT
TGTTGCAAGACATGTAGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGTTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGCTGTGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCTAAAAACAAGGCAAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTACGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGATTTCATTATCGCCGCT
TCCAATCACTAGATGAAGATTTTGGACAAACTGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCTCTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAGCTAAGAGACATGGTAGAGGCAGTGTATGT
CTTTCTAGAAGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCGAGGCTTGAGGCGGAAGAAGCAGGCAGGTTAGCTGAAG
CGATGCAGCATGGGCAAGTGTGGGATGCTGTCGTTCAATTGATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTC
TCATTTCCATTATTTCAACAGATGATGGATACAGGACTGTCCTCTCTCAAGTTCGCTTTGATTCCCCCTTCACTTGATCA
AGTGTTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTGTCATTGGAGTAAATGATGGGGTTA
TACCTGCACGGCCCTCTGATGAAAGTGTATTGTCTGAAGACGACCGGGAATGGTTAAAACGGGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTGGATGAACAATTTTTGATATATCAAGCGCTGTCCAGTCCATCGCACTACCTGTATTT
ATCCTATTCAGCTTCAGATACGGAAGGGCGGTCTCTTTTGCCTTCGACCCTCATCAAGTATTGTGAGGAACTGATGCCAA
ACCATCAGCAAGCCCTCTATGTGTTAGATCCAGAGCAGTTGGATGATCAGGAGCAATTAAAATTTGTAACAAACGAGCAT
GTCTCATTATCCTATACCGTTTCGCAGCTGCAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCCAACCGCGACGTATCAAAAAAAATCATGTCCAGCTTGTTCTTTACAAATAGAGCGAAAC
GTTTAAATCCGAATGTCACAAAAGAACTTTATGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGCTAAAACTAAAAGATCGACAATTTTACAAATTAGAAGCACCTGATATCGG
CCAGCTCTTTCACTCGGCTTTAAAACTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTGATCTACTCAAGGCATGCAATTGAACAGCTGGCTCCACGTCTGCAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATAAAAGAAAAGCTTCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGATCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCTTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGGAGGCGACACCGGCAGGAATTTTATACTTCCATATTCATGACC
CGCTGATTCAAGCCCCTATTCCGCTTGGAGAAGATGCAATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAGGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACAAGTCATCCAAGCTGGTTT
GAAAAAGGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAGGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAGATAAACCCATATAAATTAAAGGATCAAACCCCT
TGCCGCTTTTGTTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCACCTCTGA
GAAGGATGATGTCATGTTAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.581

99.316

0.661

Multiple sequence alignment