Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CYAN7822_RS00175 Genome accession   NC_014501
Coordinates   43787..44890 (+) Length   367 a.a.
NCBI ID   WP_013320200.1    Uniprot ID   E0UHE9
Organism   Gloeothece verrucosa PCC 7822     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 38787..49890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYAN7822_RS00155 (Cyan7822_0033) - 38915..39373 (-) 459 WP_013320195.1 orange carotenoid protein N-terminal domain-containing protein -
  CYAN7822_RS00160 (Cyan7822_0035) - 39817..40614 (-) 798 WP_013320197.1 CPBP family intramembrane glutamic endopeptidase -
  CYAN7822_RS00165 (Cyan7822_0036) - 40678..41946 (-) 1269 WP_013320198.1 ATP-binding protein -
  CYAN7822_RS00170 (Cyan7822_0037) - 42012..43298 (-) 1287 WP_013320199.1 serine/threonine-protein kinase -
  CYAN7822_RS00175 (Cyan7822_0038) pilM 43787..44890 (+) 1104 WP_013320200.1 type IV pilus biogenesis protein PilM Machinery gene
  CYAN7822_RS00180 (Cyan7822_0039) - 44896..45696 (+) 801 WP_013320201.1 PilN domain-containing protein -
  CYAN7822_RS00185 (Cyan7822_0040) pilO 45693..46451 (+) 759 WP_013320202.1 type 4a pilus biogenesis protein PilO -
  CYAN7822_RS00190 (Cyan7822_0041) - 46563..48749 (+) 2187 WP_013320203.1 secretin and TonB N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40955.01 Da        Isoelectric Point: 4.3456

>NTDB_id=38368 CYAN7822_RS00175 WP_013320200.1 43787..44890(+) (pilM) [Gloeothece verrucosa PCC 7822]
MLSFLKKLLPKQSQGFGMEITPERVNLAQLSKQGQNYRLSKFCTVEIPEGIFQEGKIVDSPALAEIIQEMLTEYKIKAKR
VATGVPMRESIIRIIPIPAELDDQELRDMVLNHEASLYLPYPREEVDLDYHKLGYFEDEDGIEKVHVLLVATRREVTDSY
IETFQQAGLQVDVIEINSFALIRTIREQLRQFSSNEAAVLVDIEFDNTEIAIVVDGVPQFSRTVPIGTYQLQNALSRAMN
LPTSRSPDILQGMTIPVTAFDSMSTAGAAINPGMTALMRVLGELTDELRRSINFYLNQSDELEIVQLLLAGPGGGLAQLD
EFFTQRLSIPTMQIDPITALSLDVEQEITSVQRPGLGTVLGLGLREV

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=38368 CYAN7822_RS00175 WP_013320200.1 43787..44890(+) (pilM) [Gloeothece verrucosa PCC 7822]
ATGTTAAGTTTTTTGAAAAAACTGCTTCCTAAGCAATCACAAGGATTTGGGATGGAAATCACCCCAGAGCGAGTAAATCT
AGCACAACTATCTAAACAGGGACAAAATTATCGACTGTCCAAATTTTGTACAGTAGAAATTCCTGAAGGAATCTTCCAAG
AGGGAAAAATCGTGGATTCTCCAGCTTTAGCGGAAATCATCCAAGAGATGTTGACCGAGTATAAGATTAAAGCCAAGCGA
GTAGCCACAGGAGTACCGATGCGGGAGTCAATTATCCGTATTATTCCCATTCCCGCAGAACTCGACGATCAAGAATTACG
GGATATGGTACTCAATCATGAAGCAAGTTTATATTTACCTTATCCCAGAGAAGAAGTAGACCTTGATTATCATAAGCTCG
GCTATTTTGAAGACGAAGATGGCATTGAAAAAGTTCACGTGCTGCTGGTAGCAACGCGACGGGAAGTGACCGACTCTTAC
ATAGAAACATTTCAGCAAGCGGGTTTACAAGTGGATGTGATAGAAATTAATAGTTTTGCCCTTATTCGTACCATTAGAGA
ACAACTAAGACAATTTAGTTCCAATGAAGCGGCCGTATTGGTGGATATTGAATTTGACAATACGGAAATAGCCATTGTCG
TGGATGGAGTGCCACAATTTTCTCGAACAGTGCCCATCGGAACTTATCAGTTGCAAAATGCTCTCTCACGTGCAATGAAT
TTACCCACCTCGAGAAGTCCTGATATTCTTCAAGGAATGACCATTCCCGTCACGGCATTTGATAGTATGAGTACCGCAGG
AGCGGCGATTAACCCAGGCATGACCGCATTAATGAGAGTTTTAGGCGAACTAACCGATGAATTACGTCGTTCGATCAACT
TTTATCTCAATCAGAGCGACGAATTAGAAATAGTACAACTCTTGTTGGCCGGGCCCGGGGGAGGACTTGCCCAATTAGAT
GAATTTTTTACTCAGCGCTTAAGTATCCCGACGATGCAGATCGATCCGATCACGGCTCTTTCTTTAGATGTTGAGCAAGA
GATTACCTCAGTTCAACGACCTGGGTTAGGAACGGTATTGGGTTTAGGATTGCGAGAGGTGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0UHE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

78.142

99.728

0.779

Multiple sequence alignment