Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   SP670_RS04990 Genome accession   NC_014498
Coordinates   935120..935968 (+) Length   282 a.a.
NCBI ID   WP_000705306.1    Uniprot ID   A0A098Z7D2
Organism   Streptococcus pneumoniae 670-6B     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 930120..940968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP670_RS04965 (SP670_1004) - 930835..931542 (+) 708 WP_000638482.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  SP670_RS04970 (SP670_1005) - 931547..932569 (+) 1023 WP_000609868.1 ribitol-5-phosphate dehydrogenase -
  SP670_RS04975 (SP670_1006) licA 932586..933455 (+) 870 WP_000411207.1 choline kinase LicA -
  SP670_RS04980 (SP670_1007) - 933445..934323 (+) 879 WP_000837610.1 DMT family transporter -
  SP670_RS04985 (SP670_1008) - 934335..935024 (+) 690 WP_000643970.1 sugar phosphate nucleotidyltransferase -
  SP670_RS04990 (SP670_1009) dprA/cilB/dalA 935120..935968 (+) 849 WP_000705306.1 DNA-processing protein DprA Machinery gene
  SP670_RS14210 (SP670_1010) - 936152..936499 (-) 348 WP_013315439.1 hypothetical protein -
  SP670_RS05000 (SP670_1012) - 937077..938119 (+) 1043 Protein_962 DUF389 domain-containing protein -
  SP670_RS05005 (SP670_1013) topA 938286..940373 (+) 2088 WP_000246895.1 type I DNA topoisomerase -
  SP670_RS05010 (SP670_1014) - 940594..940953 (+) 360 WP_001237219.1 YbaN family protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31062.72 Da        Isoelectric Point: 5.4047

>NTDB_id=38285 SP670_RS04990 WP_000705306.1 935120..935968(+) (dprA/cilB/dalA) [Streptococcus pneumoniae 670-6B]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=38285 SP670_RS04990 WP_000705306.1 935120..935968(+) (dprA/cilB/dalA) [Streptococcus pneumoniae 670-6B]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGTCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAATCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTTTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098Z7D2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae D39

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae R6

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

100

100

1
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.582

100

0.986
  dprA/cilB/dalA Streptococcus mitis SK321

97.872

100

0.979
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.714

99.291

0.553
  dprA Staphylococcus aureus N315

37.809

100

0.379
  dprA Haemophilus influenzae Rd KW20

38.202

94.681

0.362

Multiple sequence alignment