Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   P411_RS16655 Genome accession   NZ_CP050998
Coordinates   3300230..3300994 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli O39:NM str. F8704-2     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3295230..3305994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P411_RS16630 yhhJ 3297086..3298210 (+) 1125 WP_001216257.1 ABC transporter permease -
  P411_RS16635 - 3298314..3298544 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  P411_RS16640 - 3298541..3298900 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  P411_RS16645 nikR 3299020..3299421 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  P411_RS16650 nikE 3299427..3300233 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  P411_RS16655 amiE 3300230..3300994 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  P411_RS16660 nikC 3300994..3301827 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  P411_RS16665 nikB 3301824..3302768 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  P411_RS16670 nikA 3302768..3304342 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  P411_RS16675 acpT 3304453..3305040 (-) 588 WP_000285783.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=382428 P411_RS16655 WP_001136236.1 3300230..3300994(-) (amiE) [Escherichia coli O39:NM str. F8704-2]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=382428 P411_RS16655 WP_001136236.1 3300230..3300994(-) (amiE) [Escherichia coli O39:NM str. F8704-2]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment