Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HB725_RS27725 Genome accession   NZ_CP050335
Coordinates   5969588..5970184 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain DVT401     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5964588..5975184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB725_RS27705 (HB725_27760) aroK 5965719..5966237 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HB725_RS27710 (HB725_27765) pilQ 5966249..5968393 (-) 2145 WP_174816580.1 type 4a pilus secretin PilQ Machinery gene
  HB725_RS27715 (HB725_27770) comL/pilP 5968447..5968971 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HB725_RS27720 (HB725_27775) pilO 5968968..5969591 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB725_RS27725 (HB725_27780) pilN 5969588..5970184 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HB725_RS27730 (HB725_27785) pilM 5970184..5971248 (-) 1065 WP_126475841.1 type IV pilus assembly protein PilM Machinery gene
  HB725_RS27735 (HB725_27790) - 5971433..5973904 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=379415 HB725_RS27725 WP_003095839.1 5969588..5970184(-) (pilN) [Pseudomonas aeruginosa strain DVT401]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=379415 HB725_RS27725 WP_003095839.1 5969588..5970184(-) (pilN) [Pseudomonas aeruginosa strain DVT401]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCGCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAAATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369