Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HB725_RS25080 Genome accession   NZ_CP050335
Coordinates   5380599..5381024 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain DVT401     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5375599..5386024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB725_RS25065 (HB725_25110) pilX 5376163..5376750 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  HB725_RS25070 (HB725_25115) pilY1 5376762..5380253 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  HB725_RS25075 (HB725_25120) pilY2 5380255..5380602 (+) 348 WP_012614546.1 type 4a fimbrial biogenesis protein PilY2 -
  HB725_RS25080 (HB725_25125) comF 5380599..5381024 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HB725_RS25085 (HB725_25130) ispH 5381071..5382015 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HB725_RS25090 (HB725_25135) fkpB 5382101..5382541 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HB725_RS25095 (HB725_25140) lspA 5382534..5383043 (-) 510 WP_003094728.1 signal peptidase II -
  HB725_RS25100 (HB725_25145) ileS 5383036..5385867 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=379407 HB725_RS25080 WP_003094721.1 5380599..5381024(+) (comF) [Pseudomonas aeruginosa strain DVT401]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=379407 HB725_RS25080 WP_003094721.1 5380599..5381024(+) (comF) [Pseudomonas aeruginosa strain DVT401]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383