Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HB727_RS25860 Genome accession   NZ_CP050334
Coordinates   5639517..5640113 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain DVT410     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5634517..5645113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB727_RS25840 (HB727_25890) aroK 5635648..5636166 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HB727_RS25845 (HB727_25895) pilQ 5636178..5638322 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  HB727_RS25850 (HB727_25900) comL/pilP 5638376..5638900 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HB727_RS25855 (HB727_25905) pilO 5638897..5639520 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB727_RS25860 (HB727_25910) pilN 5639517..5640113 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HB727_RS25865 (HB727_25915) pilM 5640113..5641177 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HB727_RS25870 (HB727_25920) - 5641362..5643833 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=379314 HB727_RS25860 WP_003095839.1 5639517..5640113(-) (pilN) [Pseudomonas aeruginosa strain DVT410]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=379314 HB727_RS25860 WP_003095839.1 5639517..5640113(-) (pilN) [Pseudomonas aeruginosa strain DVT410]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369