Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HB727_RS23240 Genome accession   NZ_CP050334
Coordinates   5055140..5055565 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain DVT410     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5050140..5060565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB727_RS23225 (HB727_23265) pilX 5050704..5051291 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  HB727_RS23230 (HB727_23270) pilY1 5051303..5054794 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  HB727_RS23235 (HB727_23275) pilY2 5054796..5055143 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  HB727_RS23240 (HB727_23280) comF 5055140..5055565 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HB727_RS23245 (HB727_23285) ispH 5055612..5056556 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HB727_RS23250 (HB727_23290) fkpB 5056642..5057082 (-) 441 WP_023108763.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HB727_RS23255 (HB727_23295) lspA 5057075..5057584 (-) 510 WP_003102615.1 signal peptidase II -
  HB727_RS23260 (HB727_23300) ileS 5057577..5060408 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=379306 HB727_RS23240 WP_003094721.1 5055140..5055565(+) (comF) [Pseudomonas aeruginosa strain DVT410]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=379306 HB727_RS23240 WP_003094721.1 5055140..5055565(+) (comF) [Pseudomonas aeruginosa strain DVT410]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383