Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HB739_RS08100 Genome accession   NZ_CP050326
Coordinates   1764394..1764819 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain DVT423     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1759394..1769819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB739_RS08080 (HB739_08110) ileS 1759551..1762382 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  HB739_RS08085 (HB739_08115) lspA 1762375..1762884 (+) 510 WP_003102615.1 signal peptidase II -
  HB739_RS08090 (HB739_08120) fkpB 1762877..1763317 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HB739_RS08095 (HB739_08125) ispH 1763403..1764347 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HB739_RS08100 (HB739_08130) comF 1764394..1764819 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HB739_RS08105 (HB739_08135) pilY2 1764816..1765163 (-) 348 WP_012614546.1 type 4a fimbrial biogenesis protein PilY2 -
  HB739_RS08110 (HB739_08140) pilY1 1765165..1768656 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  HB739_RS08115 (HB739_08145) pilX 1768668..1769255 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=378562 HB739_RS08100 WP_003094721.1 1764394..1764819(-) (comF) [Pseudomonas aeruginosa strain DVT423]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=378562 HB739_RS08100 WP_003094721.1 1764394..1764819(-) (comF) [Pseudomonas aeruginosa strain DVT423]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383