Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HB744_RS05100 Genome accession   NZ_CP050323
Coordinates   1078160..1078756 (+) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain DVT429     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073160..1083756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB744_RS05090 (HB744_05100) - 1074440..1076911 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  HB744_RS05095 (HB744_05105) pilM 1077096..1078160 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HB744_RS05100 (HB744_05110) pilN 1078160..1078756 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HB744_RS05105 (HB744_05115) pilO 1078753..1079376 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB744_RS05110 (HB744_05120) comL/pilP 1079373..1079897 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HB744_RS05115 (HB744_05125) pilQ 1079951..1082095 (+) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  HB744_RS05120 (HB744_05130) aroK 1082107..1082625 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=378242 HB744_RS05100 WP_003095839.1 1078160..1078756(+) (pilN) [Pseudomonas aeruginosa strain DVT429]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=378242 HB744_RS05100 WP_003095839.1 1078160..1078756(+) (pilN) [Pseudomonas aeruginosa strain DVT429]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAAATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ATAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369