Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   HB747_RS25270 Genome accession   NZ_CP050322
Coordinates   5443984..5444409 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain DVT729     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5438984..5449409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB747_RS25255 (HB747_25300) pilX 5439548..5440135 (+) 588 WP_023114842.1 type 4a pilus minor pilin PilX -
  HB747_RS25260 (HB747_25305) pilY1 5440147..5443638 (+) 3492 WP_023114843.1 type 4a pilus biogenesis protein PilY1 -
  HB747_RS25265 (HB747_25310) pilY2 5443640..5443987 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  HB747_RS25270 (HB747_25315) comF 5443984..5444409 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  HB747_RS25275 (HB747_25320) ispH 5444456..5445400 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HB747_RS25280 (HB747_25325) fkpB 5445486..5445926 (-) 441 WP_023114844.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HB747_RS25285 (HB747_25330) lspA 5445919..5446428 (-) 510 WP_003102615.1 signal peptidase II -
  HB747_RS25290 (HB747_25335) ileS 5446421..5449252 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=378188 HB747_RS25270 WP_003094721.1 5443984..5444409(+) (comF) [Pseudomonas aeruginosa strain DVT729]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=378188 HB747_RS25270 WP_003094721.1 5443984..5444409(+) (comF) [Pseudomonas aeruginosa strain DVT729]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCAGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383