Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HAV35_RS10025 Genome accession   NZ_CP050120
Coordinates   1987709..1988884 (-) Length   391 a.a.
NCBI ID   WP_010887416.1    Uniprot ID   Q9RW99
Organism   Deinococcus radiodurans strain BND-54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1982709..1993884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAV35_RS10010 (HAV35_10015) - 1984440..1986347 (-) 1908 WP_010887419.1 hypothetical protein -
  HAV35_RS10015 (HAV35_10020) pilO 1986344..1987003 (-) 660 WP_010887418.1 type II secretion system protein M -
  HAV35_RS10020 (HAV35_10025) - 1987000..1987716 (-) 717 WP_010887417.1 hypothetical protein -
  HAV35_RS10025 (HAV35_10030) pilM 1987709..1988884 (-) 1176 WP_010887416.1 type IV pilus biogenesis protein PilM Machinery gene
  HAV35_RS10030 (HAV35_10035) - 1989164..1989967 (-) 804 WP_010887415.1 hypothetical protein -
  HAV35_RS10035 (HAV35_10040) murF 1990002..1991303 (-) 1302 WP_010887414.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
  HAV35_RS10040 (HAV35_10045) - 1991331..1991810 (-) 480 WP_231885322.1 short-chain dehydrogenase -
  HAV35_RS10045 (HAV35_10050) - 1991837..1993165 (-) 1329 WP_227086001.1 PQQ-binding-like beta-propeller repeat protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 42173.15 Da        Isoelectric Point: 4.6785

>NTDB_id=376837 HAV35_RS10025 WP_010887416.1 1987709..1988884(-) (pilM) [Deinococcus radiodurans strain BND-54]
MTSLINRFRSRSAAIGVEIGTSTIKVVALKAGAPPSLQHAVMVPTPIGSMRDGLVVEPQAVASELKSLLAEHRITTRHAV
TAVPNQVAVTRNIMVPRMDRKDLQSAIRWEAERYIPYPIDEVTLDFDLLDDPANVPEDGQMEVVIAAAPTEAVHRQIEVL
RLAGLEPTVVDLKSFAALRALRGNLLGEHLTKSTLTGTNYTEAGEVALVMEIGASSSVINLVRGDRILMTRNINVSADDF
TTALQKAFDLDFAAAEDVKLGYATATTPTEDEEDLLNFDLSREQYSPARVFEVVRPVLGDLITEIRRSLEFYRVQSGDVV
IDRTFLAGGGAKLRGLAAAIGDALGFGVEVASPWLTVQTDQAGVDTGYLQTNAPEFTVPLGLALRGVMGRG

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=376837 HAV35_RS10025 WP_010887416.1 1987709..1988884(-) (pilM) [Deinococcus radiodurans strain BND-54]
ATGACGAGCCTCATTAACCGTTTTCGCAGTCGTTCCGCCGCCATCGGCGTGGAGATCGGCACCAGCACCATCAAGGTGGT
GGCCCTTAAGGCAGGGGCCCCGCCTTCCCTGCAACACGCGGTGATGGTCCCCACCCCCATCGGCAGCATGCGTGACGGTC
TGGTCGTCGAACCCCAGGCAGTGGCGAGCGAACTCAAAAGCCTGCTGGCCGAGCACCGCATCACCACCCGCCACGCCGTC
ACCGCCGTGCCCAATCAGGTGGCCGTGACCCGCAACATCATGGTGCCGCGCATGGACCGCAAGGACCTGCAAAGCGCCAT
TCGCTGGGAAGCCGAGCGCTACATCCCTTACCCCATCGACGAGGTCACGCTCGACTTCGACCTGCTCGACGACCCCGCCA
ACGTGCCCGAGGACGGGCAGATGGAAGTGGTCATCGCGGCGGCGCCCACCGAGGCCGTGCACCGTCAGATCGAGGTGCTG
CGCCTCGCGGGCCTCGAACCCACCGTGGTGGACCTCAAGAGCTTCGCGGCGCTGCGGGCGCTACGCGGCAACCTGCTCGG
CGAGCACCTCACCAAGAGCACCCTAACCGGCACCAACTACACCGAGGCCGGCGAGGTGGCGCTGGTGATGGAAATCGGCG
CGAGCAGCAGCGTCATCAATCTGGTGCGCGGCGACCGCATCCTGATGACCCGCAACATCAACGTGTCCGCCGACGATTTC
ACCACTGCGCTGCAAAAGGCCTTCGACCTCGACTTTGCCGCCGCCGAGGATGTCAAGCTCGGTTACGCCACCGCCACCAC
CCCCACCGAGGACGAGGAAGACCTGCTCAACTTCGACCTCAGCCGCGAGCAGTACAGCCCGGCGCGCGTGTTCGAAGTGG
TGCGCCCGGTGCTCGGCGACCTGATCACCGAAATCCGCCGCTCGCTGGAGTTCTACCGCGTGCAGAGCGGCGACGTGGTC
ATCGACCGGACCTTCCTCGCCGGGGGCGGCGCCAAGCTGCGCGGCCTGGCGGCGGCCATCGGGGACGCGCTGGGCTTCGG
GGTCGAAGTCGCGTCGCCCTGGCTGACGGTGCAGACCGACCAGGCGGGCGTGGACACCGGCTACCTCCAGACCAACGCCC
CCGAATTCACCGTGCCGCTGGGACTGGCGCTGCGGGGGGTGATGGGCCGTGGTTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-260)

Type IV pilus assembly protein PilM; (PilM_2)

(291-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RW99

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

46.512

98.977

0.46