Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HAV23_RS06760 Genome accession   NZ_CP050116
Coordinates   1341543..1342718 (+) Length   391 a.a.
NCBI ID   WP_010887416.1    Uniprot ID   Q9RW99
Organism   Deinococcus radiodurans strain BNK-50     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1336543..1347718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAV23_RS06740 (HAV23_06740) - 1337263..1338591 (+) 1329 WP_227086001.1 PQQ-binding-like beta-propeller repeat protein -
  HAV23_RS06745 (HAV23_06745) - 1338618..1339097 (+) 480 WP_231885322.1 short-chain dehydrogenase -
  HAV23_RS06750 (HAV23_06750) murF 1339125..1340425 (+) 1301 Protein_1330 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
  HAV23_RS06755 (HAV23_06755) - 1340460..1341263 (+) 804 WP_010887415.1 hypothetical protein -
  HAV23_RS06760 (HAV23_06760) pilM 1341543..1342718 (+) 1176 WP_010887416.1 type IV pilus biogenesis protein PilM Machinery gene
  HAV23_RS06765 (HAV23_06765) - 1342767..1343426 (+) 660 WP_243760087.1 hypothetical protein -
  HAV23_RS06770 (HAV23_06770) pilO 1343423..1344082 (+) 660 WP_010887418.1 type II secretion system protein M -
  HAV23_RS06775 (HAV23_06775) - 1344079..1345986 (+) 1908 WP_010887419.1 hypothetical protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 42173.15 Da        Isoelectric Point: 4.6785

>NTDB_id=376771 HAV23_RS06760 WP_010887416.1 1341543..1342718(+) (pilM) [Deinococcus radiodurans strain BNK-50]
MTSLINRFRSRSAAIGVEIGTSTIKVVALKAGAPPSLQHAVMVPTPIGSMRDGLVVEPQAVASELKSLLAEHRITTRHAV
TAVPNQVAVTRNIMVPRMDRKDLQSAIRWEAERYIPYPIDEVTLDFDLLDDPANVPEDGQMEVVIAAAPTEAVHRQIEVL
RLAGLEPTVVDLKSFAALRALRGNLLGEHLTKSTLTGTNYTEAGEVALVMEIGASSSVINLVRGDRILMTRNINVSADDF
TTALQKAFDLDFAAAEDVKLGYATATTPTEDEEDLLNFDLSREQYSPARVFEVVRPVLGDLITEIRRSLEFYRVQSGDVV
IDRTFLAGGGAKLRGLAAAIGDALGFGVEVASPWLTVQTDQAGVDTGYLQTNAPEFTVPLGLALRGVMGRG

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=376771 HAV23_RS06760 WP_010887416.1 1341543..1342718(+) (pilM) [Deinococcus radiodurans strain BNK-50]
ATGACGAGCCTCATTAACCGTTTTCGCAGTCGTTCCGCCGCCATCGGCGTGGAGATCGGCACCAGCACCATCAAGGTGGT
GGCCCTTAAGGCAGGGGCCCCGCCTTCCCTGCAACACGCGGTGATGGTCCCCACCCCCATCGGCAGCATGCGTGACGGTC
TGGTCGTCGAACCCCAGGCAGTGGCGAGCGAACTCAAAAGCCTGCTGGCCGAGCACCGCATCACCACCCGCCACGCCGTC
ACCGCCGTGCCCAATCAGGTGGCCGTGACCCGCAACATCATGGTGCCGCGCATGGACCGCAAGGACCTGCAAAGCGCCAT
TCGCTGGGAAGCCGAGCGCTACATCCCTTACCCCATCGACGAGGTCACGCTCGACTTCGACCTGCTCGACGACCCCGCCA
ACGTGCCCGAGGACGGGCAGATGGAAGTGGTCATCGCGGCGGCGCCCACCGAGGCCGTGCACCGTCAGATCGAGGTGCTG
CGCCTCGCGGGCCTCGAACCCACCGTGGTGGACCTCAAGAGCTTCGCGGCGCTGCGGGCGCTACGCGGCAACCTGCTCGG
CGAGCACCTCACCAAGAGCACCCTAACCGGCACCAACTACACCGAGGCCGGCGAGGTGGCGCTGGTGATGGAAATCGGCG
CGAGCAGCAGCGTCATCAATCTGGTGCGCGGCGACCGCATCCTGATGACCCGCAACATCAACGTGTCCGCCGACGATTTC
ACCACTGCGCTGCAAAAGGCCTTCGACCTCGACTTTGCCGCCGCCGAGGATGTCAAGCTCGGTTACGCCACCGCCACCAC
CCCCACCGAGGACGAGGAAGACCTGCTCAACTTCGACCTCAGCCGCGAGCAGTACAGCCCGGCGCGCGTGTTCGAAGTGG
TGCGCCCGGTGCTCGGCGACCTGATCACCGAAATCCGCCGCTCGCTGGAGTTCTACCGCGTGCAGAGCGGCGACGTGGTC
ATCGACCGGACCTTCCTCGCCGGGGGCGGCGCCAAGCTGCGCGGCCTGGCGGCGGCCATCGGGGACGCGCTGGGCTTCGG
GGTCGAAGTCGCGTCGCCCTGGCTGACGGTGCAGACCGACCAGGCGGGCGTGGACACCGGCTACCTCCAGACCAACGCCC
CCGAATTCACCGTGCCGCTGGGACTGGCGCTGCGGGGGGTGATGGGCCGTGGTTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-260)

Type IV pilus assembly protein PilM; (PilM_2)

(291-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RW99

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

46.512

98.977

0.46